Cat #: BLP-CL011
Size: 40 µg
Type: Synthetic peptide
Form: Lyophilized powder
Applications: wb, ihc
Application key:
WB- Western blot, IHC- Immunohistochemistry
For research purposes only. not for human use
Properties
Sequence
- (C)EYVKRKQRYEVDFNLE, corresponding to amino acid residues 679-694 of mouse Anoctamin-1 (Accession Q8BHY3).
Accession (Uniprot) Number Q8BHY3
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
Applications
Demonstration of Pre-adsorption control
- Western blot analysis of rat dorsal root ganglion (DRG) lysate:1. Anti-TMEM16A (ANO1) (extracellular) Antibody (#ACL-011), (1:200).
2. Anti-TMEM16A (ANO1) (extracellular) Antibody, preincubated with TMEM16A/ANO1 (extracellular) Blocking Peptide (#BLP-CL011). - Western blot analysis of rat small intestine lysate:1. Anti-TMEM16A (ANO1) (extracellular) Antibody (#ACL-011), (1:200).
2. Anti-TMEM16A (ANO1) (extracellular) Antibody, preincubated with TMEM16A/ANO1 (extracellular) Blocking Peptide (#BLP-CL011). - Mouse tongue sections (1:50), (Cherkashin, A.P. et al. (2016) Pflugers Arch. 468, 305.).
Last update: 13/02/2024