Overview
Type: Synthetic peptide
Form: Lyophilized powder
TMEM16B/ANO2 (extracellular) Blocking Peptide (#BLP-CL012) is the original antigen used for immunization during Anti-TMEM16B (ANO2) (extracellular) Antibody (#ACL-012) generation. The blocking peptide binds and ‘blocks’ Anti-TMEM16B/ANO2 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat brain lysate:1. Anti-TMEM16B (ANO2) (extracellular) Antibody (#ACL-012), (1:200).
2. Anti-TMEM16B (ANO2) (extracellular) Antibody, preincubated with TMEM16B/ANO2 (extracellular) Blocking Peptide (#BLP-CL012).
Expression of Anoctamin-2 in mouse olfactory bulbImmunohistochemical staining of mouse olfactory bulb using Anti-TMEM16B (ANO2) (extracellular) Antibody (#ACL-012), (1:200). A. Anoctamin-2 is detected in anoctamin-positive axons (arrows). B. Staining with mouse anti-synaptophysin (red) and DAPI (blue) reveals the glomeruli of the olfactory bulb (asterisk). C. Merge of the above images reveals the spatial relationship of the anotctamin-2 positive axons to the olfactory bulb organization.
Properties
Sequence
- (C)HSKRPEQWDLDHSLE, corresponding to amino acid residues 632-646 of mouse Anoctamin-2 (Accession Q8CFW1).
Accession (Uniprot) Number Q8CFW1
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
