Overview
Type: Synthetic peptide
Form: Lyophilized Powder
TMEM175 Blocking Peptide (#BLP-PC175) is the original antigen used for immunization during Anti-TMEM175 Antibody (#APC-175) generation. The blocking peptide binds and ‘blocks’ Anti-TMEM175 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat spleen membranes (lanes 1 and 3) and mouse spleen lysates (lanes 2 and 4):1-2. Anti-TMEM175 Antibody (#APC-175), (1:200).
3-4. Anti-TMEM175 Antibody, preincubated with TMEM175 Blocking Peptide (BLP-PC175).
Western blot analysis of human SH-SY5Y neuroblastoma cell line lysate (lanes 1 and 4), human THP-1 monocytic leukemia cell line lysate (lanes 2 and 5) and human LNCaP prostate adenocarcinoma cell line lysate (lanes 3 and 6):1-3. Anti-TMEM175 Antibody (#APC-175), (1:200).
4-6. Anti-TMEM175 Antibody, preincubated with TMEM175 Blocking Peptide (BLP-PC175).
Expression of TMEM175 in rat medial septum.Immunohistochemical staining of perfusion-fixed frozen rat brain sections using Anti-TMEM175 Antibody (#APC-175), (1:300), followed by goat anti-rabbit-AlexaFluor-488. A. TMEM175 immunoreactivity (green) appears in cells of the medial septum (arrows). B. Pre-incubation of the antibody with TMEM175 Blocking Peptide (BLP-PC175), suppressed staining. Cell nuclei are stained with DAPI (blue).
Expression of TMEM175 in mouse piriform cortex.Immunohistochemical staining of perfusion-fixed frozen mouse brain sections using Anti-TMEM175 Antibody (#APC-175), (1:300), followed by goat anti-rabbit-AlexaFluor-488. A. TMEM175 immunoreactivity (green) appears in cells of the piriform cortex (arrows). B. Pre-incubation of the antibody with TMEM175 Blocking Peptide (BLP-PC175), suppressed staining. Cell nuclei are stained with DAPI (blue).
Properties
Sequence
- Peptide CEDNVPDPKDVQEK, corresponding to amino acid residues 278 - 291 of mouse TMEM175 (Accession Q9CXY1).
Accession (Uniprot) Number Q9CXY1
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
