Overview
Type: Synthetic peptide
Form: Lyophilized powder
TREM2 (extracellular) Blocking Peptide (#BLP-NR018) is the original antigen used for immunization during Anti-TREM2 (extracellular) Antibody (#ANR-018) generation. The blocking peptide binds and ‘blocks’ Anti-TREM2 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of mouse (lanes 1 and 3) and rat (lanes 2 and 4) brain lysates:1,2. Anti-TREM2 (extracellular) Antibody (#ANR-018), (1:200).
3,4. Anti-TREM2 (extracellular) Antibody, preincubated with TREM2 (extracellular) Blocking Peptide (#BLP-NR018).
Western blot analysis of mouse BV-2 microglia (lanes 1 and 4), human U-87 MG glioblastoma (lanes 2 and 5) and human THP-1 monocytic leukemia (lanes 3 and 6) cell line lysates:1-3. Anti-TREM2 (extracellular) Antibody (#ANR-018), (1:200).
4-6. Anti-TREM2 (extracellular) Antibody, preincubated with TREM2 (extracellular) Blocking Peptide (#BLP-NR018).
Expression of TREM2 in mouse hippocampus in a kainic acid neurodegeneration model.Immunohistochemical staining of perfusion-fixed frozen mouse brain sections collected 4 days post kainate injection with Anti-TREM2 (extracellular) Antibody (#ANR-018), (1:300), followed by goat anti-rabbit-AlexaFluor-488. A. Staining in the mouse hippocampal CA1 region showed TREM2 immunoreactivity (green) in astrocytes in the stratum oriens (SO) and the stratum radiatum (SR). B. Pre-incubation of the antibody with TREM2 (extracellular) Blocking Peptide (BLP-NR018), suppressed staining. Cell nuclei are stained with DAPI (blue).
Properties
Sequence
- (C)KHWGRRKAW(S)RQLGE, corresponding to amino acid residues 42-56 of mouse TREM2 (Accession Q99NH8).
Accession (Uniprot) Number Q99NH8
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
