Overview
Type: Synthetic peptide
Form: Lyophilized powder
TRIC-B/TMEM38B Blocking Peptide (#BLP-TC001) is the original antigen used for immunization during Anti-TRIC-B (TMEM38B) Antibody (#ATC-001) generation. The blocking peptide binds and ‘blocks’ Anti-TRIC-B/TMEM38B primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat brain (lanes 1 and 4), mouse brain (lanes 2 and 5) and human SH-SY5Y brain neuroblastoma (lanes 3 and 6) lysates:1-3. Anti-TRIC-B (TMEM38B) Antibody (#ATC-001), (1:200).
4-6. Anti-TRIC-B (TMEM38B) Antibody, preincubated with TRIC-B/TMEM38B Blocking Peptide (#BLP-TC001).
Expression of TRIC-B in rat testisImmunohistochemical staining of rat testis paraffin-embedded sections using Anti-TRIC-B (TMEM38B) Antibody (#ATC-001), followed by goat anti-rabbit-Alexa-Fluor-594 secondary antibody. A. TRIC-B labeling appears in the columnar principal epithelium of the epididymis and in the endothelium of the surrounding blood vessels. B. Nuclear staining using DAPI as the counterstain. C. Merged images of A and B.
Properties
Sequence
- (C)GMKEVTRTWKIVG, corresponding to amino acid residues 116-126 of rat TRIC-B (Accession Q68FV1).
Accession (Uniprot) Number Q68FV1
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 2 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
