Overview
Type: Synthetic peptide
Form: Lyophilized powder
TrkB (extracellular) Blocking Peptide (#BLP-NT019) is the original antigen used for immunization during Anti-TrkB (extracellular) Antibody (#ANT-019) generation. The blocking peptide binds and ‘blocks’ Anti-TrkB (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat brain membranes (lanes 1 and 3) and HEK-TrkB transfected cell lysates (lanes 2 and 4):1,2. Anti-TrkB (extracellular) Antibody (#ANT-019), (1:200).
3,4. Anti-TrkB (extracellular) Antibody, preincubated with TrkB (extracellular) Blocking Peptide (#BLP-NT019).
Western blot analysis of mouse brain lysates:1. Anti-TrkB (extracellular) Antibody (#ANT-019), (1:200).
2. Anti-TrkB (extracellular) Antibody, preincubated with TrkB (extracellular) Blocking Peptide (#BLP-NT019).
Expression of TrkB in rat DRGImmunohistochemical staining of rat dorsal root ganglia (DRG) frozen sections using Anti-TrkB (extracellular) Antibody (#ANT-019), (1:100). TrkB (red) is expressed in DRG neurons. Hoechst 33342 is used as the counterstain.
Properties
Sequence
- (C)RLEPNSIDPENITE, corresponding to amino acid residues 57-70 of rat TrkB (Accession Q63604).
Accession (Uniprot) Number Q63604
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
