Overview
Lyophilized Powder yes
Source Synthetic
MW: 273.4
Purity: >99%
Effective concentration 1 nM - 1 µM.
Structure
Chemical name (8-methyl-8-azabicyclo[3.2.1]octan-3-yl) 3,5-dimethylbenzoate.
Molecular formula C17H23NO2.
CAS No.: 85181-40-4
PubChem CID 2837530
Activity Tropanyl-3,5-dimethylbenzoate is a 5-HT3 receptor antagonist1.
References-Activity
- Yang, N. et al. (2010) Br. J. Pharmacol. 159, 1623.
Shipping and storage Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Solubility 50-100 mM in DMSO. Centrifuge all product preparations before use (10000 x g 5 min).
Storage of solutions Up to four weeks at 4°C or three months at -20°C.
Our bioassay
Alomone Labs Tropanyl-3,5-dimethylbenzoate citrate blocks 5-HT3A receptors expressed in HEK 293T cells.5-HT3A receptor currents were elicited with 10 µM 5-HT, delivered every 3 minutes. Tropanyl-3,5-dimethylbenzoate (#T-135) was applied 30 seconds before stimulation at 1, 10 and 50 µM as indicated and completely inhibited the 5-HT induced current in a dose dependent and reversible manner.
Scientific background
Serotonin (5-hydroxytryptamine, 5-HT) is a monoamine neurotransmitter synthesized from the amino acid Tryptophan by the enzyme tryptophan-decarboxylase. The 5-HT3 channel is a ligand-gated ion channel receptor of the cys-loop channel family. It is a pentamer with five subunits surrounding a central channel1.
Tropanyl-3,5-dimethylbenzoate is a synthetic, potent and selective antagonist of the 5-HT3 receptor. Tropanyl-3,5-dimethylbenzoate has an effective concentration of 1 nM – 1 μM. Interestingly, in rat mucosa and submucosa preparations Tropanyl causes an increase of 5-HT induced Δ/sc (change in short circuit current) and causes a decline of Δ/sc in rat intact colon suggesting a double role for 5-HT3 receptors in this tissue2.
Target 5-HT3 receptors
Lyophilized Powder
For research purposes only, not for human use
Last Update: 07/05/2024
Specifications
Citations
Citations
