Overview
Alternative Name Mydriacyl®
Lyophilized Powder yes
Source Synthetic
MW: 284.3
Purity: >96%.
Effective concentration 10 nM - 100 µM.
Structure
Chemical name N-Ethyl-3-hydroxy-2-phenyl-N-(pyridinylmethyl)propanamide.
Molecular formula C17H20N2O2.
CAS No.: 1508-75-4
Activity Tropicamide is a muscarinic receptor (mAChR) antagonist, which shows a 3 fold selectivity for M4 over other muscarinic receptors1-2. Tropicamide inhibits porcine intravesical ureter contraction with pIC50 of ~7.53.
References-Activity
- Liao, C.F. et al. (1989) J. Biol. Chem. 264, 7328.
- Lazareno, S. et al. (1990) Br. J. Pharmacol. 109, 1120.
- Hernandez, M. et al. (1993) Br. J. Pharmacol. 110, 1413.
Shipping and storage Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Solubility DMSO. Centrifuge all product preparations before use (10000 x g 5 min).
Storage of solutions Up to four weeks at 4°C or three months at -20°C.
Our bioassay
Alomone Labs Tropicamide inhibits carbachol’s effect on M1 mAChR expressed in C6 cells.Cells were loaded with Fluo-3 AM and changes in intracellular Ca2+ were detected via changes in Fluo-3 emission. A. Normalized fluorescence before and after application (at 20 seconds, see arrow) of 10 μM Carbachol following a 20 min incubation with 0-100 μM Tropicamide (#T-120) (as indicated). B. Inhibition (as percent of control) of 10 µM Carbachol evoked Ca2+ rise, plotted against Tropicamide concentrations.
Target M1-M5 muscarinic receptors
Lyophilized Powder
For research purposes only, not for human use
Last Update: 30/03/2021
Specifications
Citations
Citations
