Overview
Type: Synthetic peptide
Form: Lyophilized powder
TROY/TNFRSF19 (extracellular) Blocking Peptide (#BLP-NT033) is the original antigen used for immunization during Anti-TROY (TNFRSF19) (extracellular) Antibody (#ANT-033) generation. The blocking peptide binds and ‘blocks’ Anti-TROY/TNFRSF19 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of human U-87 MG glioblastoma cell line lysate (lanes 1 and 3) and human Malme-3M melanoma cell line lysate (lanes 2 and 4):1,2. Anti-TROY (TNFRSF19) (extracellular) Antibody (#ANT-033), (1:400).
3,4. Anti-TROY (TNFRSF19) (extracellular) Antibody, preincubated with TROY/TNFRSF19 (extracellular) Blocking Peptide (#BLP-NT033).
Western blot analysis of new born rat brain membranes (lanes 1 and 3) and mouse brain membranes (lanes 2 and 4):1,2. Anti-TROY (TNFRSF19) (extracellular) Antibody (#ANT-033), (1:400).
3,4. Anti-TROY (TNFRSF19) (extracellular) Antibody, preincubated with TROY/TNFRSF19 (extracellular) Blocking Peptide (#BLP-NT033).
Properties
Sequence
- CRPHRFKEDWGFQK, corresponding to amino acid residues 75-88 of mouse TROY (Accession Q9JLL3).
Accession (Uniprot) Number Q9JLL3
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
