Overview
Type: Synthetic peptide
Form: Lyophilized powder
TRPA1 (extracellular) Blocking Peptide (#BLP-CC037) is the original antigen used for immunization during Anti-TRPA1 (extracellular) Antibody (#ACC-037) generation. The blocking peptide binds and ‘blocks’ Anti-TRPA1 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat DRG (lanes 1,2), non-differentiated PC12 cells (lanes 3,5) and differentiated PC12 cells (lanes 4,6) lysates:1,3,4. Anti-TRPA1 (extracellular) Antibody (#ACC-037), (1:200).
2,5,6. Anti-TRPA1 (extracellular) Antibody, preincubated with TRPA1 (extracellular) Blocking Peptide (#BLP-CC037).
Expression of TRPA1 in rat DRGImmunohistochemical staining of rat dorsal root ganglion (DRG) using Anti-TRPA1 (extracellular) Antibody (#ACC-037) (1:200). A. TRPA1 staining (green) is detected in both cells (horizontal arrow) and processes (vertical arrow). B. Nuclear staining using DAPI as the counterstain. C. Merged image of A and B.- Human urethra (1:500) (Gratzke, C. et al. (2009) Eur. Urol. 55, 696.).
Western blot analysis of rat brain lysate (lanes 1 and 3) and mouse brain lysate (lanes 2 and 4):1-2. Guinea Pig Anti-TRPA1 (extracellular) Antibody (#ACC-037-GP), (1:200).
3-4. Guinea Pig Anti-TRPA1 (extracellular) Antibody (#ACC-037-GP), preincubated with TRPA1 (extracellular) Blocking Peptide (#BLP-CC037)
Expression of TRPA1 in mouse brainstem.Immunohistochemical staining of perfusion-fixed frozen mouse brain sections with Guinea Pig Anti-TRPA1 (extracellular) Antibody (#ACC-037-GP), (1:1000), followed by goat anti-guinea pig-Alexa-594. A. TRPA1 immunoreactivity (red) appears in the wall of the fourth cerebral ventricle (arrows). B. Pre-incubation of the antibody with TRPA1 (extracellular) Blocking Peptide (#BLP-CC037), suppressed staining. Cell nuclei are stained with DAPI (blue).
Expression of TRPA1 in rat spinal cord.Immunohistochemical staining of perfusion-fixed frozen rat spinal cord sections with Guinea Pig Anti-TRPA1 (extracellular) Antibody (#ACC-037-GP), (1:1000), followed by goat anti-guinea pig-Alexa-594. A. TRPA1 immunoreactivity (red) appears in laminae 1-2 of the dorsal horn of the spinal cord (arrows). B. Pre-incubation of the antibody with TRPA1 (extracellular) Blocking Peptide (#BLP-CC037), suppressed staining. Cell nuclei are stained with DAPI (blue).
Properties
Sequence
- (C)NSTGIINETSDHSE, corresponding to amino acid residues 747-760 of human TRPA1 (Accession O75762).
Accession (Uniprot) Number O75762
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 μg peptide per 1 μg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
