Size: 40 µg
Type: Synthetic peptide
Form: Lyophilized powder
Applications: wb, ihc
For research purposes only. not for human use
Properties
Sequence
- (C)NSTGIINETSDHSE, corresponding to amino acid residues 747-760 of human TRPA1 (Accession O75762).
Accession (Uniprot) Number O75762
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 μg peptide per 1 μg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat DRG (lanes 1,2), non-differentiated PC12 cells (lanes 3,5) and differentiated PC12 cells (lanes 4,6) lysates:1,3,4. Anti-TRPA1 (extracellular) Antibody (#ACC-037), (1:200).
2,5,6. Anti-TRPA1 (extracellular) Antibody, preincubated with TRPA1 (extracellular) Blocking Peptide (#BLP-CC037).- Mouse and rat DRG frozen sections (1:200). Human urethra (1:500) (Gratzke, C. et al. (2009) Eur. Urol. 55, 696.).
Western blot analysis of rat brain lysate (lanes 1 and 3) and mouse brain lysate (lanes 2 and 4):1-2. Guinea Pig Anti-TRPA1 (extracellular) Antibody (#ACC-037-GP), (1:200).
3-4. Guinea Pig Anti-TRPA1 (extracellular) Antibody (#ACC-037-GP), preincubated with TRPA1 (extracellular) Blocking Peptide (#BLP-CC037)
Last update: 14/02/2024
