Cat #: AK-212_KIT
Size: 24 Vials
Form: Lyophilized
Application key:
CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot
Species reactivity key:
H- Human, M- Mouse, R- Rat
Anti-TRPC1 Antibody Cat #: ACC-010
Size:
50 µl
Host: Rabbit
Type: Polyclonal
Application:
IC, IF, IH, IP, WB
Reactivity:
H, M, R
Immunogen
Peptide QLYDKGYTSKEQKDC, corresponding to amino acid residues 557-571 of human TRPC1 (Accession P48995). Intracellular.
Homology
Mouse, rat, human - identical; rabbit, bovine - 14/15 amino acid residues identical; Xenopus Laevis - 11/14 amino acid residues identical.
TRPC1 Blocking Peptide Cat #: BLP-CC010
Size:
40 µg
Anti-TRPC1 (extracellular) Antibody Cat #: ACC-118
Size:
50 µl
Host: Rabbit
Type: Polyclonal
Application:
IC, IF, IFC, LCI, WB
Reactivity:
H, M, R
Immunogen
Peptide (C)SMGQMLQDFGK, corresponding to amino acid residues 495-505 of rat TRPC1 (Accession Q9QX01). 2nd extracellular loop.
Homology
Human, mouse - identical.
TRPC1 (extracellular) Blocking Peptide Cat #: BLP-CC118
Size:
40 µg
Anti-TRPC2 Antibody Cat #: ACC-027
Size:
50 µl
Host: Rabbit
Type: Polyclonal
Application:
WB
Reactivity:
M, R
Immunogen
Peptide (C)KNRSGVRMFKDGD, corresponding to amino acid residues 12-24 of mouse TRPC2 (Accession Q9R244). Intracellular, N-terminus.
Homology
Rat - 12/13 amino acid residues identical.
TRPC2 Blocking Peptide Cat #: BLP-CC027
Size:
40 µg
Anti-TRPC3 Antibody Cat #: ACC-016
Size:
50 µl
Host: Rabbit
Type: Polyclonal
Application:
IC, IF, IFC, IH, IP, WB
Reactivity:
H, M, R
Immunogen
Peptide HKLSEKLNPSVLRC, corresponding to amino acid residues 822-835 of mouse TRPC3 (Accession Q9QZC1). Intracellular, C-terminus.
Homology
Human, rat - 13/14 amino acid residues identical.
TRPC3 Blocking Peptide Cat #: BLP-CC016
Size:
40 µg
Guinea pig Anti-TRPC3 Antibody Cat #: ACC-016-GP
Size:
50 µl
Host: Guinea pig
Type: Polyclonal
Application:
IH, WB
Reactivity:
M, R
Immunogen
Peptide HKLSEKLNPSVLRC, corresponding to amino acid residues 822-835 of mouse TRPC3 (Accession Q9QZC1). Intracellular, C-terminus.
Homology
Human, rat - 13/14 amino acid residues identical.
TRPC3 Blocking Peptide Cat #: BLP-CC016
Size:
40 µg
Anti-TRPC4 Antibody Cat #: ACC-018
Size:
50 µl
Host: Rabbit
Type: Polyclonal
Application:
IC, IF, IH, IP, WB
Reactivity:
H, M, R
Immunogen
Peptide (C)KEKHAHEEDSSIDYDL, corresponding to amino acid residues 943-958 of mouse TRPC4 (Accession Q9QUQ5). Intracellular, C-terminus.
Homology
Rat, human - 15/16 amino acid residues identical; bovine - 11/15 amino acid residues identical.
TRPC4 Blocking Peptide Cat #: BLP-CC018
Size:
40 µg
Anti-TRPC4 (extracellular) Antibody Cat #: ACC-119
Size:
50 µl
Host: Rabbit
Type: Polyclonal
Application:
IC, IF, IH, LCI, WB
Reactivity:
H, M, R
Immunogen
Peptide (C)KYSALNPRESWD, corresponding to amino acid residues 458-469 of rat TRPC4 (Accession O35119). 2nd extracellular loop.
Homology
Mouse, human - identical.
TRPC4 (extracellular) Blocking Peptide Cat #: BLP-CC119
Size:
40 µg
Anti-TRPC5 Antibody Cat #: ACC-020
Size:
50 µl
Host: Rabbit
Type: Polyclonal
Application:
IC, IF, IH, IP, WB
Reactivity:
H, M, R
Immunogen
Peptide (C)HKWGDGQEEQVTTRL, corresponding to amino acid residues 959-973 of human TRPC5 (Accession Q9UL62). Intracellular, C-terminus.
Homology
Rat, mouse, rabbit - identical.
TRPC5 Blocking Peptide Cat #: BLP-CC020
Size:
40 µg
Anti-TRPC6 Antibody Cat #: ACC-017
Size:
50 µl
Host: Rabbit
Type: Polyclonal
Application:
IC, IF, IFC, IH, IP, WB
Reactivity:
H, M, R
Immunogen
Peptide (C)RRNESQDYLLMDELG, corresponding to amino acid residues 24-38 of mouse TRPC6 (Accession Q61143). Intracellular, N-terminus.
Homology
Rat - identical; human - 15/16 amino acid residues identical.
TRPC6 Blocking Peptide Cat #: BLP-CC017
Size:
40 µg
Guinea pig Anti-TRPC6 Antibody Cat #: ACC-017-GP
Size:
50 µl
Host: Guinea pig
Type: Polyclonal
Application:
IF, IH, WB
Reactivity:
M, R
Immunogen
Peptide (C)RRNESQDYLLMDELG, corresponding to amino acid residues 24-38 of mouse TRPC6 (Accession Q61143). Intracellular, N-terminus.
Homology
Rat - identical; human - 15/16 amino acid residues identical.
TRPC6 Blocking Peptide Cat #: BLP-CC017
Size:
40 µg
Anti-TRPC6 (extracellular) Antibody Cat #: ACC-120
Size:
50 µl
Host: Rabbit
Type: Polyclonal
Application:
IC, IF, IFC, IH, LCI, WB
Reactivity:
H, M, R
Immunogen
Peptide (C)DNVKYYNLARIKWD, corresponding to amino acid residues 573-586 of rat TRPC6 (Accession Q61143). 2nd extracellular loop.
Homology
Rat, human - identical
TRPC6 (extracellular) Blocking Peptide Cat #: BLP-CC120
Size:
40 µg
Anti-TRPC7 (extracellular) Antibody Cat #: ACC-066
Size:
50 µl
Host: Rabbit
Type: Polyclonal
Application:
IC, IF, LCI, WB
Reactivity:
H, M, R
Immunogen
Peptide (C)DQHVQDDTLHNVS, corresponding to amino acid residues 504-516 of human TRPC7 (Accession Q9HCX4). 2nd extracellular loop.
Homology
Rat, mouse - 11/13 amino acid residues identical.
TRPC7 (extracellular) Blocking Peptide Cat #: BLP-CC066
Size:
40 µg
Antibody Storage & Reconstitution Instructions
Storage before reconstitution
Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Reconstitution
Add deionized water, depending on the sample size.
Storage after reconstitution
The reconstituted solution can be stored at 4°C for up to 2 weeks. For longer periods, small aliquots should be stored at -20°C or below.
Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).
Control antigen storage before reconstitution
Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Control antigen reconstitution
100 μl water.
Control antigen storage after reconstitution
-20ºC.
Preadsorption control
1 μg peptide per 1 μg antibody.
Note
Guinea pig polyclonal antibodies (#ACC-016-GP & #ACC-017-GP) are included in this Explorer Kit. Please take into account when reacting with a secondary antibody.
For research purposes only, not for human use
