Overview
Type: Synthetic peptide
Form: Lyophilized powder
TRPC3 Blocking Peptide (#BLP-CC016) is the original antigen used for immunization during Anti-TRPC3 Antibody (#ACC-016) generation. The blocking peptide binds and ‘blocks’ Anti-TRPC3 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat heart membranes:1. Anti-TRPC3 Antibody (#ACC-016), (1:200).
2. Anti-TRPC3 Antibody, preincubated with TRPC3 Blocking Peptide (#BLP-CC016).
Expression of TRPC3 in rat pituitary glandImmunohistochemical staining of rat pituitary gland paraffin embedded sections using Anti-TRPC3 Antibody (#ACC-016), (1:100). TRPC3 is mainly expressed in the adenohypophysis (on left). Hematoxilin is used as the counterstain.
Expression of TRPC3 in mouse cerebellumImmunohistochemical staining of mouse cerebellum using Anti-TRPC3 Antibody (#ACC-016) (A). Immunoreactivity appears in the molecular layer and in Purkinje cells (B).
Expression of TRPC3 in rat cerebellumImmunohistochemical staining of TRPC3 in rat cerebellum using Anti-TRPC3 Antibody (#ACC-016). A. TRPC3 (green) appears in Purkinje cells (arrows) including both soma and dendrites and as well as in the molecular layer neuropil (asterisk). Staining of the same section with mouse anti-parvalbumin (red) reveals that TRPC3 is not expressed in molecular layer interneurons. B. Pre-incubating Anti-TRPC3 with the TRPC3 peptide antigen blocks staining. DAPI is used as the counterstain (blue).
Multiplex staining of TRPC6 and TRPC3 in rat cerebellumImmunohistochemical staining of rat cerebellum frozen section using Guinea pig Anti-TRPC6 Antibody (#ACC-017-GP) and rabbit Anti-TRPC3 Antibody (#ACC-016). A. TRPC6 staining (green) appears in molecular layer and in Purkinje cells. B. In the same section, staining of TRPC3 (red) appears as well in both molecular layer and Purkinje cells. C. Merge images of A and B indicates extensive co-localization. DAPI is used as the counterstain (blue).
Western blot analysis of rat brain lysate:1. Guinea pig Anti-TRPC3 Antibody (#ACC-016-GP), (1:400).
2. Guinea pig Anti-TRPC3 Antibody preincubated with TRPC3 Blocking Peptide (#BLP-CC016).
Expression of TRPC3 in mouse cerebellum.Immunohistochemical staining of perfusion-fixed frozen mouse brain sections with Guinea pig Anti-TRPC3 Antibody (#ACC-016-GP), (1:200), followed by goat anti-guinea pig-AlexaFluor-594. A. TRPC3 immunoreactivity (red) appears in purkinje cells (arrows). B. Pre-incubation of the antibody with TRPC3 Blocking Peptide (#BLP-CC016), suppressed staining. Cell nuclei are stained with DAPI (blue).
Expression of TRPC3 in rat cerebellum.Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Guinea pig Anti-TRPC3 Antibody (#ACC-016-GP), (1:200), followed by goat anti-guinea pig-AlexaFluor-594. A. TRPC3 immunoreactivity (red) appears in purkinje cells (arrows). B. Pre-incubation of the antibody with TRPC3 Blocking Peptide (#BLP-CC016), suppressed staining. Cell nuclei are stained with DAPI (blue).
Properties
Sequence
- HKLSEKLNPSVLRC, corresponding to amino acid residues 822-835 of mouse TRPC3 (Accession Q9QZC1).
Accession (Uniprot) Number Q9QZC1
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
