Overview
Type: Synthetic peptide
Form: Lyophilized powder
TRPC4 (extracellular) Blocking Peptide (#BLP-CC119) is the original antigen used for immunization during Anti-TRPC4 (extracellular) Antibody (#ACC-119) generation. The blocking peptide binds and ‘blocks’ Anti-TRPC4 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat brain lysates (lanes 1 and 3) and mouse brain lysates (lanes 2 and 4):1-2. Anti-TRPC4 (extracellular) Antibody (#ACC-119), (1:200).
3-4. Anti-TRPC4 (extracellular) Antibody, preincubated with TRPC4 (extracellular) Blocking Peptide (#BLP-CC119).
Expression of TRPC4 in rat brainImmunohistochemical staining of rat third ventricle region using Anti-TRPC4 (extracellular) Antibody (#ACC-119). A. TRPC4 staining (red) is detected along the wall of the third ventricle (horizontal arrow) and astrocytic fibers extending from the wall (vertical arrow). B. Nucleus staining using DAPI as the counterstain (blue). C. Merge images of A and B.
Properties
Sequence
- (C)KYSALNPRESWD, corresponding to amino acid residues 458-469 of rat TRPC4 (Accession O35119).
Accession (Uniprot) Number O35119
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
