Overview
Type: Synthetic peptide
Form: Lyophilized powder
TRPC4 Blocking Peptide (#BLP-CC018) is the original antigen used for immunization during Anti-TRPC4 Antibody (#ACC-018) generation. The blocking peptide binds and ‘blocks’ Anti-TRPC4 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat brain membranes:1. Anti-TRPC4 Antibody (#ACC-018), (1:200).
2. Anti-TRPC4 Antibody, preincubated with TRPC4 Blocking Peptide (#BLP-CC018).
Western blot analysis of human PC3 Caucasian prostate adenocarcinoma (lanes 1 and 3) and human LNCaP prostate carcinoma (lanes 2 and 4) cell lysates:1,3. Anti-TRPC4 Antibody (#ACC-018), (1:200).
2,4. Anti-TRPC4 Antibody, preincubated with TRPC4 Blocking Peptide (#BLP-CC018).
Expression of TRPC4 in mouse cerebellumImmunohistochemical staining of mouse cerebellum frozen sections with Anti-TRPC4 Antibody (#ACC-018). A. TRPC4 (red) appears in Purkinje cells (arrows) and in the molecular (Mol) layer. B. Staining with mouse anti-parvalbumin (PV) in the same brain section. C. Confocal merge of TRPC4 and PV demonstrates partial co-localization in the Purkinje and the molecular layers.
Properties
Sequence
- (C)KEKHAHEEDSSIDYDL, corresponding to amino acid residues 943-958 of mouse TRPC4 (Accession Q9QUQ5).
Accession (Uniprot) Number Q9QUQ5
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 μg peptide per 1 μg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
