Overview
Type: Synthetic peptide
Form: Lyophilized powder
TRPC5 Blocking Peptide (#BLP-CC020) is the original antigen used for immunization during Anti-TRPC5 Antibody (#ACC-020) generation. The blocking peptide binds and ‘blocks’ Anti-TRPC5 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat brain membranes:1. Anti-TRPC5 Antibody (#ACC-020), (1:200).
2. Anti-TRPC5 Antibody, preincubated with TRPC5 Blocking Peptide (#BLP-CC020).
Expression of TRPC5 in rat cortexImmunohistochemical staining of rat cortex using Anti-TRPC5 Antibody (#ACC-020). A. TRPC5 immunoreactivity (green) appears in pyramidal shaped neuronal soma (vertical arrows) and apical dendrites (horizontal arrows). B. Nuclear staining using DAPI as the counterstain (blue). C. Merged image of A and B.- Rat substantia nigra (De March, Z. et al. (2006) Neurosci. Lett. 402, 35.).
Properties
Sequence
- (C)HKWGDGQEEQVTTRL, corresponding to amino acid residues 959-973 of human TRPC5 (Accession Q9UL62).
Accession (Uniprot) Number Q9UL62
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
