Overview
Type: Synthetic peptide
Form: Lyophilized powder
TRPC6 (extracellular) Blocking Peptide (#BLP-CC120) is the original antigen used for immunization during Anti-TRPC6 (extracellular) Antibody (#ACC-120) generation. The blocking peptide binds and ‘blocks’ Anti-TRPC6 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat brain lysates:1. Anti-TRPC6 (extracellular) Antibody (#ACC-120), (1:200).
2. Anti-TRPC6 (extracellular) Antibody, preincubated with TRPC6 (extracellular) Blocking Peptide (#BLP-CC120).- Rat kidney sections (1:50) (Ilatovskaya, D.V. et al. (2015) Sci. Rep. 5, 17637.).
Properties
Sequence
- (C)DNVKYYNLARIKWD, corresponding to amino acid residues 573-586 of rat TRPC6 (Accession Q61143).
Accession (Uniprot) Number Q61143
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
