Overview
Type: Synthetic peptide
Form: Lyophilized powder
TRPC6 Blocking Peptide (#BLP-CC017) is the original antigen used for immunization during Anti-TRPC6 Antibody (#ACC-017) generation. The blocking peptide binds and ‘blocks’ Anti-TRPC6 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat brain membranes:1. Anti-TRPC6 Antibody (#ACC-017), (1:200).
2. Anti-TRPC6 Antibody, preincubated with TRPC6 Blocking Peptide (#BLP-CC017).
Expression of TRPC6 in mouse cerebellumImmunohistochemical staining of mouse cerebellum using Anti-TRPC6 Antibody (#ACC-017). A. TRPC6 (red) appears in Purkinje cells (arrows) and in the molecular (Mol) layer. B. Staining with mouse anti parvalbumin in the same brain section. C. Confocal merge of TRPC6 and PV demonstrates partial co-localization in the Purkinje and the molecular layers.
Western blot analysis of rat brain membranes:1. Guinea pig Anti-TRPC6 Antibody (#ACC-017-GP), (1:200).
2. Guinea pig Anti-TRPC6 Antibody, preincubated with TRPC6 Blocking Peptide (#BLP-CC017).
Expression of TRPC6 in rat cerebellumImmunohistochemical staining of rat cerebellum using Guinea pig Anti-TRPC6 Antibody (#ACC-017-GP). A. TRPC6 (green) appears in the soma of Purkinje cells (white arrow). B. Parvalbumin (red), a marker of Purkinje and interneuronal cells, is stained in the same section. C. Merge of the images demonstrates intense expression of TRPC6 in the soma of Purkinje cells and lower expression in the molecular (Mol) layer. DAPI is used as the counterstain (blue).
Multiplex staining of TRPC6 and TRPC3 in rat cerebellumImmunohistochemical staining of rat cerebellum frozen section using Guinea pig Anti-TRPC6 Antibody (#ACC-017-GP) and rabbit Anti-TRPC3-ATTO Fluor-594 Antibody (#ACC-016-AR). A. TRPC6 staining (green) appears in molecular layer and in Purkinje cells. B. In the same section as in A, staining of TRPC3 (red) appears as well in both molecular layer and Purkinje cells. C. Merge images of A and B indicates co-localization in Purkinje cells and molecular layer.
Multiplex staining of TRPC6 and TRPC3 in rat cerebellumImmunohistochemical staining of rat cerebellum frozen section using Guinea pig Anti-TRPC6 Antibody (#ACC-017-GP) and rabbit Anti-TRPC3 Antibody (#ACC-016). A. TRPC6 staining (green) appears in molecular layer and in Purkinje cells. B. In the same section, staining of TRPC3 (red) appears as well in both molecular layer and Purkinje cells. C. Merge images of A and B indicates extensive co-localization. DAPI is used as the counterstain (blue).
Properties
Sequence
- (C)RRNESQDYLLMDELG, corresponding to amino acid residues 24-38 of mouse TRPC6 (Accession Q61143).
Accession (Uniprot) Number Q61143
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
