Size: 40 µg
Type: Synthetic peptide
Form: Lyophilized powder
Applications: wb, ihc
For research purposes only. not for human use
Properties
Sequence
- (C)RRNESQDYLLMDELG, corresponding to amino acid residues 24-38 of mouse TRPC6 (Accession Q61143).
Accession (Uniprot) Number Q61143
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat brain membranes:1. Anti-TRPC6 Antibody (#ACC-017), (1:200).
2. Anti-TRPC6 Antibody, preincubated with TRPC6 Blocking Peptide (#BLP-CC017).- Mouse brain sections.
Western blot analysis of rat brain membranes:1. Guinea pig Anti-TRPC6 Antibody (#ACC-017-GP), (1:200).
2. Guinea pig Anti-TRPC6 Antibody, preincubated with TRPC6 Blocking Peptide (#BLP-CC017).- Rat cerebellum floating frozen section (1:100).
- Following a broad screen of secondary antibodies, the following was used for this application: #106-485-006 (Jackson ImmunoResearch).
Last update: 02/01/2024
