Overview
Type: Synthetic peptide
Form: Lyophilized powder
TRPM2 (extracellular) Blocking Peptide (#BLP-CC128) is the original antigen used for immunization during Anti-TRPM2 (extracellular) Antibody (#ACC-128) generation. The blocking peptide binds and ‘blocks’ Anti-TRPM2 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of mouse brain lysate (lanes 1 and 3) and rat brain lysate (lanes 2 and 4):1, 2. Anti-TRPM2 (extracellular) Antibody (#ACC-128), (1:200).
3, 4. Anti-TRPM2 (extracellular) Antibody, preincubated with TRPM2 (extracellular) Blocking Peptide (#BLP-CC128).
Western blot analysis of human THP-1 monocytic leukemia cell line lysate (lanes 1 and 3) and human HL-60 promyelocytic leukemia cell line lysate (lanes 2 and 4):1, 2. Anti-TRPM2 (extracellular) Antibody (#ACC-128), (1:200).
3, 4. Anti-TRPM2 (extracellular) Antibody, preincubated with TRPM2 (extracellular) Blocking Peptide (#BLP-CC128).
Expression of TRPM2 in rat substantia nigraImmunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-TRPM2 (extracellular) Antibody (#ACC-128), followed by goat anti-rabbit-AlexaFluor-488. TRPM2 immunoreactivity (green) appears in neurons in the substantia nigra pars compacta (SNC), (arrows). SNR=substantia nigra pars reticulata. Cell nuclei are stained with DAPI (blue).
Properties
Sequence
- (C)NGTDPYKPK(S)PESD, corresponding to amino acid residues 999 - 1012 of human TRPM2 (Accession O94759).
Accession (Uniprot) Number O94759
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
