Cat #: BLP-CC050
Size: 40 µg
Type: Synthetic peptide
Form: Lyophilized powder
Applications: wb, ihc
Application key:
WB- Western blot, IHC- Immunohistochemistry
For research purposes only. not for human use
Properties
Sequence
- KNKDDMPYMSQAQEIH(C), corresponding to amino acid residues 816-831 of human TRPM3 (Accession Q9HCF6).
Accession (Uniprot) Number Q9HCF6
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
Applications
Demonstration of Pre-adsorption control
- Western blot analysis of rat brain membrane (lanes 1 and 4), rat brain lysate (lanes 2 and 5) and mouse brain lysate (lanes 3 and 6):1-3. Anti-TRPM3 (extracellular) Antibody (#ACC-050), (1:200).
4-6. Anti-TRPM3 (extracellular) Antibody, preincubated with TRPM3 (extracellular) Blocking Peptide (#BLP-CC050). - Mouse optic nerves sections (Papanikolaou, M. et al. (2017) Brain Struct. Funct. 222, 2993.).
Last update: 02/01/2024