Overview
Type: Synthetic peptide
Form: Lyophilized powder
TRPM5 Blocking Peptide (#BLP-CC045) is the original antigen used for immunization during Anti-TRPM5 Antibody (#ACC-045) generation. The blocking peptide binds and ‘blocks’ Anti-TRPM5 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat lung membrane (lanes 1 and 2), rat testes (lanes 3 and 4), mouse ms1 pancreas cells (lanes 5 and 7) and human LNCaP prostate cell (lanes 6 and 8) lysates:1,3,5,6. Anti-TRPM5 Antibody (#ACC-045), (1:200).
2,4,7,8. Anti-TRPM5 Antibody, preincubated with TRPM5 Blocking Peptide (#BLP-CC045).
Expression of TRPM5 in rat tongueImmunohistochemical staining of rat tongue paraffin embedded sections using Anti-TRPM5 Antibody (#ACC-045), (1:100). TRPM5 is expressed in discrete groups of cells deep in the sulcus of the papillae. Hematoxilin is used as the counterstain.
Expression of TRPM5 in rat testesImmunohistochemical staining of rat epididymis paraffin embedded sections using Anti-TRPM5 Antibody (#ACC-045), (1:100). TRPM5 is expressed in principal cells within the epididymal epithelium (arrows). Hematoxilin is used as the counterstain.
Properties
Sequence
- GSGKKRGKFVKVPS(C), corresponding to amino acid residues 32-45 of mouse TRPM5 (Accession Q9JJH7).
Accession (Uniprot) Number Q9JJH7
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 μg peptide per 1 μg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
