Overview
Type: Synthetic peptide
Form: Lyophilized powder
TRPM6 (extracellular) Blocking Peptide (#BLP-CC046) is the original antigen used for immunization during Anti-TRPM6 (extracellular) Antibody (#ACC-046) generation. The blocking peptide binds and ‘blocks’ Anti-TRPM6 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat kidney (lanes 1 and 4), rat testis (lanes 2 and 5) and mouse kidney (lanes 3 and 6) lysates:1-3. Anti-TRPM6 (extracellular) Antibody (#ACC-046), (1:200).
4-6. Anti-TRPM6 (extracellular) Antibody, preincubated with TRPM6 (extracellular) Blocking Peptide (#BLP-CC046).- Mouse placenta sections (1:50) (Suzuki, Y. et al. (2016) J. Physiol. Sci. 67, 151.).
Expression of TRPM6 in rat hippocampus.Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-TRPM6 (extracellular) Antibody (#ACC-046), (1:200), followed by goat anti-rabbit-AlexaFluor-488. A. Staining in the rat hippocampal CA1 region showed TRPM6 immunoreactivity (green) in in the pyramidal cell layer (P) and apical dendrites (arrows). B. Pre-incubation of the antibody with TRPM6 (extracellular) Blocking Peptide (BLP-CC046), suppressed staining. Cell nuclei are stained with DAPI (blue).
Properties
Sequence
- CVKDYDLERGPDEK, corresponding to amino acid residues 802-815 of mouse TRPM6 (Accession Q8CIR4).
Accession (Uniprot) Number Q8CIR4
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 μg peptide per 1 μg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
