Overview
Type: Synthetic peptide
Form: Lyophilized Powder
TRPM7 (extracellular) Blocking Peptide (#BLP-CC129) is the original antigen used for immunization during Anti-TRPM7 (extracellular) Antibody (#ACC-129) generation. The blocking peptide binds and ‘blocks’ Anti-TRPM7 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of human Jurkat T-cell leukemia cell line lysate (lanes 1 and 5), human Raji B-cell lymphoma cell line lysate (lanes 2 and 6 ), mouse WEHI B-cell lymphoma cell line lysate (lanes 3 and 7) and human NK-92 natural killer cell line lysate (lanes 4 and 8):1-4. Anti-TRPM7 (extracellular) Antibody (#ACC-129), (1:200).
5-8. Anti-TRPM7 (extracellular) Antibody, preincubated with TRPM7 (extracellular) Blocking Peptide (BLP-CC129).
Western blot analysis of rat spleen lysate:1. Anti-TRPM7 (extracellular) Antibody (#ACC-129), (1:200).
2. Anti-TRPM7 (extracellular) Antibody, preincubated with TRPM7 (extracellular) Blocking Peptide (BLP-CC129).
Expression of TRPM7 in rat parietal cortex.Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-TRPM7 (extracellular) Antibody (#ACC-129), (1:600), followed by goat anti-rabbit-AlexaFluor-488. A. TRPM7 immunoreactivity (green) appears in cortical neurons (arrows). B. Pre-incubation of the antibody with TRPM7 (extracellular) Blocking Peptide (BLP-CC129), suppressed staining. Cell nuclei are stained with DAPI (blue).
Expression of TRPM7 in rat hippocampus.Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-TRPM7 (extracellular) Antibody (#ACC-129), (1:600), followed by goat anti-rabbit-AlexaFluor-488. A. Staining in the rat hippocampal dentate gyrus, showed TRPM7 immunoreactivity (green) in neurons (arrows). B. Pre-incubation of the antibody with TRPM7 (extracellular) Blocking Peptide (BLP-CC129), suppressed staining. Cell nuclei are stained with DAPI (blue).
Properties
Sequence
- Peptide (C)RFGAKWNYINAYDNH, corresponding to amino acid residues 942 - 956 of mouse TRPM7 (Accession Q923J1).
Accession (Uniprot) Number Q923J1
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
