Name: TRPM8 Channel Basic Research Pack
Brand: Alomone Labs
SKU: ESB-301_PACK

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Cat #: ESB-301_PACK

Size: 6 Vials

Form: Lyophilized

Antibodies

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat

Anti-TRPM8 (extracellular) Antibody Cat #: ACC-049

Size: 0.2 ml Host: Rabbit Type: Polyclonal Application: IC, IF, IFC, IH, IP, LCI, N, WB Reactivity: H, M, R

Immunogen

Peptide SDVDGTTYDFAHC, corresponding to amino acid residues 917-929 of human TRPM8 (Accession Q7Z2W7). 3^rdextracellular loop.

Homology

Canis - identical; bovine - 12/13 amino acid residues identical; rat, mouse - 11/13 amino acid residues identical.

Pharmacological Reagents

Icilin Cat #: I-145

Size: 25 mg Source: Synthetic MW: 311.29 Purity: >95%

Effective concentration

1 nM – 1 µM.

Activity

Icilin is a potent TRPM8 channel activator (EC₅₀= 0.2 µM)¹.

Storage before reconstitution

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.

Reconstitution

Up to 100 mM in DMSO. Centrifuge all products before use (10000 x g 5 min).

Storage after reconstitution

Up to three months at -20°C.

WS 12 Cat #: W-105

Size: 10 mg Source: Synthetic MW: 289.41 Purity: >99%

Effective concentration

1 nM – 10 µM.

Activity

WS 12 is a TRPM8 channel activator (EC₅₀= 193 nM)¹.

Storage before reconstitution

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.

Reconstitution

Up to 75 mM in Ethanol. Centrifuge all products before use (10000 x g 5 min).

Storage after reconstitution

Up to three months at -20°C.

M8-B hydrochloride Cat #: M-220

Size: 10 mg Source: Synthetic MW: 432.96 Purity: >99%

Effective concentration

10 nM - 10 µM.

Activity

M8-B hydrochloride is a potent and selective blocker of TRPM8 channel, inhibiting rat and human TRPM8 activation by cold, by icilin and by menthol with IC₅₀values of 7.8 nM, 26.9 nM and 64.3 nM, respectively, whilst having little or no effect on other TRP channels (IC₅₀> 20 μM)¹.

Storage before reconstitution

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored desiccated at +4°C.

Reconstitution

Up to 100 mM in DMSO. Centrifuge all products before use (10000 x g 5 min).

Storage after reconstitution

Up to three months at -20°C.

TC-I 2014 Cat #: T-200

Size: 10 mg Source: Synthetic MW: 467.41 Purity: >96%

Effective concentration

1 nM – 1 µM.

Activity

TC-I 2014 is a potent and selective TRPM8 blocker, inhibiting canine, rat, and human TRPM8-mediated Ca²⁺ influx with IC₅₀ values of 0.8, 4.0, and 3.0 nM, respectively¹.

Storage before reconstitution

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at +4°C.

Reconstitution

Up to 100 mM in DMSO. Centrifuge all products before use (10000 x g 5 min).

Storage after reconstitution

Up to three months at -20°C.

Antibody Storage & Reconstitution Instructions

Storage before reconstitution

Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.

Reconstitution

Add deionized water, depending on the sample size.

Storage after reconstitution

The reconstituted solution can be stored at 4°C for up to 2 weeks. For longer periods, small aliquots should be stored at -20°C or below.
Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).

Control antigen storage before reconstitution

Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.

Control antigen reconstitution

100 μl water.

Control antigen storage after reconstitution

-20ºC.

Preadsorption control

1 μg peptide per 1 μg antibody.

For more information, please refer to individual certificate of analysis for each product.

For research purposes only, not for human use

Shipping and Ordering Information

Free International Shipping

Products are lyophilized and ship at room temperature - FREE OF CHARGE.

We ship worldwide within 24 hours of our next business day.

For more country-specific shipping and contact information see Ordering & Shipping.

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Order Summary

Certificate of Analysis

TRPM8 Channel Basic Research Pack

Antibodies

Application key:

Species reactivity key:

Anti-TRPM8 (extracellular) Antibody Cat #: ACC-049

Pharmacological Reagents

Icilin Cat #: I-145

WS 12 Cat #: W-105

M8-B hydrochloride Cat #: M-220

TC-I 2014 Cat #: T-200

Antibody Storage & Reconstitution Instructions

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