Overview
Type: Synthetic peptide
Form: Lyophilized powder
TRPM8 (extracellular) Blocking Peptide (#BLP-CC049) is the original antigen used for immunization during Anti-TRPM8 (extracellular) Antibody (#ACC-049) generation. The blocking peptide binds and ‘blocks’ Anti-TRPM8 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of prostate carcinoma cell lines DU145 (lanes 1 and 3), Human LNCaP prostate carcinoma (lanes 2 and 4) and mouse-TRPM8 transfected HEK-293 (lanes 5 and 6) cell lysates:1,2,5. Anti-TRPM8 (extracellular) Antibody (#ACC-049), (1:200).
3,4,6. Anti-TRPM8 (extracellular) Antibody, preincubated with TRPM8 (extracellular) Blocking Peptide (#BLP-CC049).
Expression of TRPM8 in rat DRGImmunohistochemical staining of rat dorsal root ganglion (DRG) frozen sections using Anti-TRPM8 (extracellular) Antibody (#ACC-049), (1:100). TRPM8 is expressed in DRG neurons. Hoechst 33342 is used as the counterstain.
Multiplex staining of TRPM8 and TrkA in rat DRGImmunohistochemical staining of perfusion-fixed frozen rat dorsal root ganglia (DRG) sections using Anti-TrkA (extracellular)-ATTO Fluor-633 Antibody (#ANT-018-FR), (1:60) and Anti-TRPM8 (extracellular) Antibody (#ACC-049), (1:300). A. TRPM8 labeling followed by goat-anti-rabbit-Alexa-488 (green). B. The same section was then labeled for TrkA (purple). C. Merge of A and B demonstrates co-localization of TRPM8 and TrkA in rat DRG (arrows). Cell nuclei were stained with DAPI (blue).
Properties
Sequence
- SDVDGTTYDFAHC, corresponding to amino acid residues 917-929 of human TRPM8 (Accession Q7Z2W7).
Accession (Uniprot) Number Q7Z2W7
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
