Overview
Type: Synthetic peptide
Form: Lyophilized powder
TRPML1/Mucolipin 1 Blocking Peptide (#BLP-CC081) is the original antigen used for immunization during Anti-TRPML1 (Mucolipin 1) Antibody (#ACC-081) generation. The blocking peptide binds and ‘blocks’ Anti-TRPML1/Mucolipin 1 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat kidney (lanes 1 and 2) and HEK-293 cells (lanes 3 and 4) lysates:1,3. Anti-TRPML1 (Mucolipin 1) Antibody (#ACC-081), (1:200).
2,4. Anti-TRPML1 (Mucolipin 1) Antibody, preincubated with TRPML1/Mucolipin 1 Blocking Peptide (#BLP-CC081).
Expression of TRPML1 in mouse hippocampus.Immunohistochemical staining of perfusion-fixed frozen mouse brain sections with Anti-TRPML1 (Mucolipin 1) Antibody (#ACC-081), (1:1000), followed by goat anti-rabbit-AlexaFluor-488. A. Staining in the CA3 hippocampal region, showed immunoreactivity (green) in pyramidal cells (vertical arrows), and in the outline of the mossy fiber terminal zone (horizontal arrows, MFT). B. Pre-incubation of the antibody with TRPML1 (Mucolipin 1) Blocking Peptide (#BLP-CC081), suppressed staining. Cell nuclei are stained with DAPI (blue). MFT= mossy fiber terminal zone.
Properties
Sequence
- (C)GRRASETERLLTPN, corresponding to amino acid residues 6-19 of mouse TRPLM1 (Accession Q99J21).
Accession (Uniprot) Number Q99J21
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
