Overview
Type: Synthetic peptide
Form: Lyophilized powder
TRPML2/Mucolipin-2 Blocking Peptide (#BLP-CC082) is the original antigen used for immunization during Anti-TRPML2 (Mucolipin-2) Antibody (#ACC-082) generation. The blocking peptide binds and ‘blocks’ Anti-TRPML2/Mucolipin-2 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of human MCF-7 breast adenocarcinoma cell line (lanes 1 and 4), rat heart (lanes 2 and 5) and mouse heart (lanes 3 and 6) lysates:1-3. Anti-TRPML2 (Mucolipin-2) Antibody (#ACC-082), (1:200).
4-6. Anti-TRPML2 (Mucolipin-2) Antibody, preincubated with TRPML2/Mucolipin-2 Blocking Peptide (#BLP-CC082).
Expression of TRPML2 in rat heartImmunohistochemical staining of rat heart paraffin-embedded sections using Anti-TRPML2 (Mucolipin-2) Antibody (#ACC-082), followed by AlexaFluor-555 secondary antibody. A. TRPML2 labeling appears specifically in the cardiac muscle fibers and in a lesser intensity in the endothelium. It does not appear in the smooth muscle fibers of blood vessels. B. Nuclear staining using DAPI as the counterstain. C. Merged images of A and B.
Properties
Sequence
- (C)RLDFYRLVQVDISFALK, corresponding to amino acid residues 212-228 of mouse TRPML2 (Accession Q8K595).
Accession (Uniprot) Number Q8K595
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
