Cat #: AK-211_KIT
Size: 26 Vials
Form: Lyophilized
Application key:
CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot
Species reactivity key:
H- Human, M- Mouse, R- Rat
Anti-TRPV1 (VR1) Antibody Cat #: ACC-030
Size:
50 µl
Host: Rabbit
Type: Polyclonal
Application:
IC, IF, IFC, IH, WB
Reactivity:
H, M, R
Immunogen
Peptide (C)EDAEVFKDSMVPGEK, corresponding to amino acid residues 824-838 of rat TRPV1 (Accession O35433). Intracellular, C-terminus.
Homology
Mouse, guinea pig - 14/15 amino acid residues identical; human - 10/15 amino acid residues identical.
TRPV1/VR1 Blocking Peptide Cat #: BLP-CC030
Size:
40 µg
Guinea pig Anti-TRPV1 (VR1) Antibody Cat #: ACC-030-GP
Size:
50 µl
Host: Guinea pig
Type: Polyclonal
Application:
IF, IH, WB
Reactivity:
R
Immunogen
Peptide (C)EDAEVFKDSMVPGEK, corresponding to amino acid residues 824-838 of rat TRPV1 (Accession O35433). Intracellular, C-terminus.
Homology
Mouse - 14/15 amino acid residues identical; human - 10/15 amino acid residues identical.
TRPV1/VR1 Blocking Peptide Cat #: BLP-CC030
Size:
40 µg
Anti-TRPV1 (VR1) (extracellular) Antibody Cat #: ACC-029
Size:
50 µl
Host: Rabbit
Type: Polyclonal
Application:
IC, IF, IFC, IH, LCI, WB
Reactivity:
M, R
Immunogen
Peptide (C)NSLPMESTPHK(S)RGS, corresponding to amino acid residues 605-619 of rat TRPV1 (Accession O35433). 3rd extracellular loop.
Homology
Rat - 14 out of 15 amino acid residues identical. Mouse - 12 out of 15 amino acid residues identical.
Won't recognize TRPV1 from human samples.
Won't recognize TRPV1 from human samples.
TRPV1/VR1 (extracellular) Blocking Peptide Cat #: BLP-CC029
Size:
40 µg
Anti-TRPV2 (VRL1) Antibody Cat #: ACC-032
Size:
50 µl
Host: Rabbit
Type: Polyclonal
Application:
IF, IH, IP, WB
Reactivity:
M, R
Immunogen
Peptide (C)KKNPTSKPGKNSASEE, corresponding to amino acid residues 735-750 of rat TRPV2 (Accession Q9WUD2). Intracellular, C-terminus.
Homology
Mouse - identical.
TRPV2/VRL1 Blocking Peptide Cat #: BLP-CC032
Size:
40 µg
Anti-TRPV2 (VRL1) (extracellular) Antibody Cat #: ACC-039
Size:
50 µl
Host: Rabbit
Type: Polyclonal
Application:
IC, IF, IFC, IH, IP, LCI, WB
Reactivity:
M, R
Immunogen
Peptide (C)HQPSLDQPAIPSSKAT, corresponding to amino acid residues 413-428 of rat TRPV2 (Accession Q9WUD2). 1st extracellular loop.
Homology
Mouse - 15/16 amino acid residues identical.
The antibody will not recognize human TRPV2.
The antibody will not recognize human TRPV2.
TRPV2/VRL1 (extracellular) Blocking Peptide Cat #: BLP-CC039
Size:
40 µg
Anti-Human TRPV2 (VRL1) (extracellular) Antibody Cat #: ACC-139
Size:
50 µl
Host: Rabbit
Type: Polyclonal
Application:
IFC, LCI, WB
Reactivity:
H
Immunogen
Peptide (C)KQAAPHLKAEVGNS, corresponding to amino acid residues 417-430 of human TRPV2 (Accession Q9Y5S1). 1st extracellular loop.
Homology
Human only.
Human TRPV2/VRL1 (extracellular) Blocking Peptide Cat #: BLP-CC139
Size:
40 µg
Guinea pig Anti-TRPV2 (VRL1) (extracellular) Antibody Cat #: ACC-039-GP
Size:
50 µl
Host: Guinea pig
Type: Polyclonal
Application:
IC, IF, IH, LCI, WB
Reactivity:
M, R
Immunogen
Peptide (C)HQPSLDQPAIPSSKAT, corresponding to amino acid residues 413-428 of rat TRPV2 (Accession Q9WUD2). 1st extracellular loop.
Homology
Mouse - 15/16.
Human - will not recognize the human epitope.
Human - will not recognize the human epitope.
TRPV2/VRL1 (extracellular) Blocking Peptide Cat #: BLP-CC039
Size:
40 µg
Anti-TRPV3 (extracellular) Antibody Cat #: ACC-033
Size:
50 µl
Host: Rabbit
Type: Polyclonal
Application:
IC, IF, IFC, IH, LCI, WB
Reactivity:
H, M, R
Immunogen
Peptide (C)REEEAIPHPLALTHK, corresponding to amino acid residues 464-478 of human TRPV3 (Accession Q8NET8). 1st extracellular loop.
Homology
Rat - 13/15 amino acid residues identical; mouse - 12/15 amino acid residues identical.
TRPV3 (extracellular) Blocking Peptide Cat #: BLP-CC033
Size:
40 µg
Anti-TRPV4 Antibody Cat #: ACC-034
Size:
50 µl
Host: Rabbit
Type: Polyclonal
Application:
IC, IF, IFC, IH, IP, WB
Reactivity:
H, M, R
Immunogen
Peptide CDGHQQGYAPKWRAEDAPL, corresponding to amino acid residues 853-871 of rat TRPV4 (Accession Q9ERZ8). Intracellular, C-terminus.
Homology
Mouse - 17/19 amino acid residues identical; human - 16/19 amino acid residues identical.
TRPV4 Blocking Peptide Cat #: BLP-CC034
Size:
40 µg
Anti-TRPV4 (extracellular) Antibody Cat #: ACC-124
Size:
50 µl
Host: Rabbit
Type: Polyclonal
Application:
IC, IF, IFC, IH, LCI, WB
Reactivity:
H, R
Immunogen
Peptide (C)EDQSN(S)TVPSYPA(S)RD, corresponding to amino acid residues 647-662 of rat TRPV4 (Accession Q9ERZ8). 3rd extracellular loop.
Homology
Rat - 14/16 amino acid residues identical; mouse - 13/16 amino acid residues identical; human - 12/16 amino acid residues identical.
TRPV4 (extracellular) Blocking Peptide Cat #: BLP-CC124
Size:
40 µg
Anti-TRPV5 Antibody Cat #: ACC-035
Size:
50 µl
Host: Rabbit
Type: Polyclonal
Application:
IC, IF, IH, WB
Reactivity:
H, M, R
Immunogen
Peptide (C)GLNLSEGDGEEVYHF, corresponding to amino acid residues 715-729 of human TRPV5 (Accession Q9NQA5). Intracellular, C-terminus.
Homology
Rat - 11/15 amino acid residues identical; rabbit - 13/15 amino acid residues identical.
TRPV5 Blocking Peptide Cat #: BLP-CC035
Size:
40 µg
Anti-TRPV6 Antibody Cat #: ACC-036
Size:
50 µl
Host: Rabbit
Type: Polyclonal
Application:
IC, IF, IH, IP, WB
Reactivity:
H, M, R
Immunogen
Peptide (C)NRGLEDGESWEYQI, corresponding to amino acid residues 712-725 of human TRPV6 (Accession Q9H1D0). Intracellular, C-terminus.
Homology
Rat, mouse - 13/14 amino acid residues identical.
TRPV6 Blocking Peptide Cat #: BLP-CC036
Size:
40 µg
Anti-Human TRPV6 (extracellular) Antibody Cat #: ACC-028
Size:
50 µl
Host: Rabbit
Type: Polyclonal
Application:
IC, IF, LCI, WB
Reactivity:
H
Immunogen
Peptide (C)RTNNRTSPRDN, corresponding to amino acid residues 395-405 of human TRPV6 (Accession Q9H1D0). 1st extracellular loop.
Human TRPV6 (extracellular) Blocking Peptide Cat #: BLP-CC028
Size:
40 µg
Antibody Storage & Reconstitution Instructions
Storage before reconstitution
Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Reconstitution
Add deionized water, depending on the sample size.
Storage after reconstitution
The reconstituted solution can be stored at 4°C for up to 2 weeks. For longer periods, small aliquots should be stored at -20°C or below.
Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).
Control antigen storage before reconstitution
Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Control antigen reconstitution
100 μl water.
Control antigen storage after reconstitution
-20ºC.
Preadsorption control
1 μg peptide per 1 μg antibody.
Note
Guinea pig polyclonal antibodies (#ACC-030-GP & #ACC-039-GP) are included in this Explorer Kit. Please take into account when reacting with a secondary antibody.
For research purposes only, not for human use
