Overview
Type: Synthetic peptide
Form: Lyophilized powder
TRPV1/VR1 Blocking Peptide (#BLP-CC030) is the original antigen used for immunization during Anti-TRPV1 (VR1) Antibody (#ACC-030) generation. The blocking peptide binds and ‘blocks’ Anti-TRPV1/VR1 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of human TRPV1 transfected HEK-293 cells:1. Anti-TRPV1 (VR1) Antibody (#ACC-030), (1:200).
2. Anti-TRPV1 (VR1) Antibody, preincubated with TRPV1/VR1 Blocking Peptide (#BLP-CC030).
Western blot analysis of rat DRG lysate:1. Anti-TRPV1 (VR1) Antibody (#ACC-030), (1:200).
2. Anti-TRPV1 (VR1) Antibody, preincubated with TRPV1/VR1 Blocking Peptide (#BLP-CC030).
Expression of TRPV1 in rat DRGImmunohistochemical staining of rat dorsal root ganglion (DRG) using Anti-TRPV1 (VR1) Antibody (#ACC-030). A. TRPV1 (red) in DRG neurons. B. Staining with mouse anti-Parvalbumin (green) in the same DRG section. C. Confocal merge of TRPV1 and Parvalbumin demonstrates colocalization (arrows).
Expression of TRPV1 in rat spinal cord dorsal horn region:Immunohistochemical staining of perfusion-fixed frozen rat spinal cord sections with Anti-TRPV1 (VR1)-ATTO Fluor-647N Antibody (#ACC-030-FRN), (1:100). A. TRPV1 immunoreactivity (purple) appears in the superficial layer of the dorsal horn (arrows). B. Pre-incubation of the antibody with TRPV1/VR1 Blocking Peptide (BLP-CC030), suppressed staining. Cell nuclei are stained with DAPI (blue).
Multiplex staining of TRPV1 and Neurokinin 1 Receptor (NK1R) in mouse dorsal raphe nucleus:Immunohistochemical staining of perfusion-fixed frozen mouse dorsal raphe nucleus sections using Anti-TRPV1 (VR1)-ATTO Fluor-647N Antibody (#ACC-030-FRN), (1:100) and Anti-Neurokinin 1 Receptor (NK1R) (extracellular)-ATTO Fluor-488 Antibody (#ATR-001-AG), (1:100). A. TRPV1 staining (purple). B. NK1 receptor staining (green). C. Merge of the two images demonstrates co-localization in the medial DRN (blue arrow) and lateral DRN (orange arrow). Cell nuclei are stained with DAPI (blue).
Western blot analysis of Rat DRG lysate:1. Guinea pig Anti-TRPV1 (VR1) Antibody (#ACC-030-GP), (1:200).
2. Guinea pig Anti-TRPV1 (VR1) Antibody preincubated with TRPV1/VR1 Blocking Peptide (#BLP-CC030).
Following a broad screen of secondary antibodies, the following were used for these applications: Western blot analysis: #106-035-006 (Jackson ImmunoResearch).
Expression of TRPV1 in rat DRGsImmunohistochemical staining of TRPV1 in rat dorsal root ganglion (DRG) using Guinea pig Anti-TRPV1 (VR1) Antibody (#ACC-030-GP), (1:200). A. TRPV1 staining (red) appears in some DRG cells (arrows). B. DAPI counterstain (blue) is used to stain nuclei. C. Merged image of panels A and B.
Properties
Sequence
- (C)EDAEVFKDSMVPGEK, corresponding to amino acid residues 824-838 of rat TRPV1 (Accession O35433).
Accession (Uniprot) Number O35433
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
