Size: 40 µg
Type: Synthetic peptide
Form: Lyophilized powder
Applications: wb, ihc
For research purposes only. not for human use
Properties
Sequence
- (C)HQPSLDQPAIPSSKAT, corresponding to amino acid residues 413-428 of rat TRPV2 (Accession Q9WUD2).
Accession (Uniprot) Number Q9WUD2
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
Applications
Demonstration of Pre-adsorption control
Western blot analysis of ND7/23 cell line membrane (lanes 1 and 3), RBL lysates (lanes 2 and 4) and rat brain membrane (lanes 5and 6):1,2,5. Anti-TRPV2 (VRL1) (extracellular) Antibody (#ACC-039), (1:200).
3,4,6. Anti-TRPV2 (VRL1) (extracellular) Antibody, preincubated with TRPV2/VRL1 (extracellular) Blocking Peptide (#BLP-CC039).- Mouse dorsal root ganglion.
Western blot analysis of rat brain (lanes 1 and 3) and rat RBL basophilic leukemia cell lysate (lanes 2 and 4):1,2. Guinea pig Anti-TRPV2 (VRL1) (extracellular) Antibody (#ACC-039-GP), (1:2000).
3,4. Guinea pig Anti-TRPV2 (VRL1) (extracellular) Antibody, preincubated with TRPV2/VRL1 (extracellular) Blocking Peptide (#BLP-CC039).- Rat dorsal root ganglia (1:60). Mouse heart sections (1:200).
Following a broad screen of secondary antibodies, the following was used for this application: #106-485-006 (Jackson ImmunoResearch).
Last update: 02/01/2024
