Overview
Type: Synthetic peptide
Form: Lyophilized powder
TRPV3 (extracellular) Blocking Peptide (#BLP-CC033) is the original antigen used for immunization during Anti-TRPV3 (extracellular) Antibody (#ACC-033) generation. The blocking peptide binds and ‘blocks’ Anti-TRPV3 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat DRG (lanes 1 and 2) and brain (lanes 3 and 4) lysates:1,3. Anti-TRPV3 (extracellular) Antibody (#ACC-033), (1:200).
2,4. Anti-TRPV3 (extracellular) Antibody, preincubated with TRPV3 (extracellular) Blocking Peptide (#BLP-CC033).
Expression of TRPV3 in mouse spinal cordImmunohistochemical staining of mouse spinal cord using Anti-TRPV3 (extracellular) Antibody (#ACC-033). A. TRPV3 (green) appears in neurons (vertical arrows) in the ventral horn of the mouse spinal cord. B. Motor neurons were stained with goat anti-choline-acetyltransferase (red). In some motor neurons (A) TRPV3 was more intense (vertical arrow) whereas in others it was weak (horizontal arrows). C. Merged images of panels A and B. The inset in C magnifies one large motor neuron (vertical arrowhead). Note the punctate pattern of TRPV3 on the surface of the soma.
Properties
Sequence
- (C)REEEAIPHPLALTHK, corresponding to amino acid residues 464-478 of human TRPV3 (Accession Q8NET8).
Accession (Uniprot) Number Q8NET8
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 μg peptide per 1 μg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
