Size: 40 µg
Type: Synthetic peptide
Form: Lyophilized powder
Applications: wb, ihc
For research purposes only. not for human use
Properties
Sequence
- CDGHQQGYAPKWRAEDAPL, corresponding to amino acid residues 853-871 of rat TRPV4 (Accession Q9ERZ8).
Accession (Uniprot) Number Q9ERZ8
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat brain lysates:1. Anti-TRPV4 Antibody (#ACC-034), (1:200).
2. Anti-TRPV4 Antibody, preincubated with TRPV4 Blocking Peptide (#BLP-CC034).
Western blot analysis of ND7/23 Mouse neuroblastoma /rat dorsal root ganglion neuron hybrid cell line lysate:1. Anti-TRPV4 Antibody (#ACC-034), (1:200).
2. Anti-TRPV4 Antibody, preincubated with TRPV4 Blocking Peptide (#BLP-CC034).- Rat brain and mouse cerebellum frozen sections.
Western blot analysis of rat brain membranes:1. Guinea pig Anti-TRPV4 Antibody (#Acc-034-GP), (1:1500).
2. Guinea pig Anti-TRPV4 Antibody, preincubated with TRPV4 Blocking Peptide (#BLP-CC034).
Last update: 02/01/2024
