Cat #: AK-223_KIT
Size: 26 Vials
Form: Lyophilized
Application key:
CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot
Species reactivity key:
H- Human, M- Mouse, R- Rat
Anti-NaV1.5 (SCN5A) (493-511) Antibody Cat #: ASC-005
Size:
50 µl
Host: Rabbit
Type: Polyclonal
Application:
IC, IF, IH, IP, WB
Reactivity:
H, M, R
Immunogen
Peptide DRLPKSDSEDGPRALNQLS(C), corresponding to amino acid residues 493-511 of rat NaV1.5 (accession number P15389). Intracellular loop between domains I and II.
Homology
Mouse - identical; human - 17/19 amino acid residues identical.
Nav1.5/SCN5A (493-511) Blocking Peptide Cat #: BLP-SC005
Size:
40 µg
Guinea pig Anti-NaV1.5 (SCN5A) Antibody Cat #: ASC-005-GP
Size:
50 µl
Host: Guinea pig
Type: Polyclonal
Application:
IF, IH, WB
Reactivity:
H, M, R
Immunogen
Peptide DRLPKSDSEDGPRALNQLSC, corresponding to amino acid residues 493-511 of rat NaV1.5 (Accession P15389). Intracellular loop between domains I and II.
Homology
Mouse - identical; human - 17/19 amino acid residues identical.
Nav1.5/SCN5A (493-511) Blocking Peptide Cat #: BLP-SC005
Size:
40 µg
Anti-NaV1.5 (SCN5A) (1978-2016) Antibody Cat #: ASC-013
Size:
50 µl
Host: Rabbit
Type: Polyclonal
Application:
IC, IFC, IH, WB
Reactivity:
H, R
Immunogen
GST fusion protein with amino acid residues 1978-2016 of human NaV1.5 (Accession Q14524), (MW: 33 kDa.). Intracellular, C-terminus.
Homology
Rat - 36/39 amino acid residues identical; mouse - 34/39 amino acid residues identical.
Nav1.5/SCN5A (1978-2016) Blocking Peptide Cat #: BLP-SC013
Size:
0.15 mg
Anti-NaV1.8 (SCN10A) Antibody Cat #: ASC-016
Size:
50 µl
Host: Rabbit
Type: Polyclonal
Application:
IC, IF, IH, IP, WB
Reactivity:
M, R
Immunogen
Peptide (C)EDEVAAKEGNSPGPQ, corresponding to amino acid residues 1943-1956 of rat NaV1.8 (Accession Q63554). Intracellular, C-terminus.
Homology
Mouse - 14/15 amino acid residues identical; human - 7/15 amino acid residues identical.
Nav1.8/SCN10A Blocking Peptide Cat #: BLP-SC016
Size:
40 µg
Guinea pig Anti-NaV1.8 (SCN10A) Antibody Cat #: ASC-016-GP
Size:
50 µl
Host: Guinea pig
Type: Polyclonal
Application:
IF, IH, WB
Reactivity:
R
Immunogen
Peptide (C)EDEVAAKEGNSPGPQ, corresponding to amino acid residues 1943-1956 of rat NaV1.8 (Accession Q63554). Intracellular, C-terminus.
Homology
Mouse - 14/15 amino acid residues identical; human - 7/15 amino acid residues identical.
Nav1.8/SCN10A Blocking Peptide Cat #: BLP-SC016
Size:
40 µg
Anti-NaV1.8 (SCN10A) (extracellular) Antibody Cat #: ASC-028
Size:
50 µl
Host: Rabbit
Type: Polyclonal
Application:
WB
Reactivity:
M, R
Immunogen
Peptide (C)EDNYLEEKPRVKSVLE, corresponding to amino acid residues 1173-1188 of rat NaV1.8 (Accession Q62968). 1st extracellular loop, domain III.
Homology
Mouse - identical; human – Not recommended for use with human samples.
Nav1.8/SCN10A (extracellular) Blocking Peptide Cat #: BLP-SC028
Size:
40 µg
Anti-SCN11A (NaV1.9) Antibody Cat #: ASC-017
Size:
50 µl
Host: Rabbit
Type: Polyclonal
Application:
IC, IF, IH, WB
Reactivity:
H, M, R
Immunogen
Peptide CNGDLSSLDVAKVKVHND, corresponding to amino acid residues 1748-1765 of rat NaV1.9 (Accession O88457). Intracellular, C-terminus.
Homology
Mouse - 15/18 amino acid residues identical; human - 14/18 amino acid residues identical.
SCN11A/Nav1.9 Blocking Peptide Cat #: BLP-SC017
Size:
40 µg
Guinea pig Anti-SCN11A (NaV1.9) Antibody Cat #: ASC-017-GP
Size:
50 µl
Host: Guinea pig
Type: Polyclonal
Application:
IF, IH, WB
Reactivity:
R
Immunogen
Peptide CNGDLSSLDVAKVKVHND, corresponding to amino acid residues 1748-1765 of rat NaV1.9 (Accession O88457). Intracellular, C-terminus.
Homology
Mouse - 15/18 amino acid residues identical; human - 14/18 amino acid residues identical.
SCN11A/Nav1.9 Blocking Peptide Cat #: BLP-SC017
Size:
40 µg
Anti-Pan NaV Antibody Cat #: ASC-003
Size:
50 µl
Host: Rabbit
Type: Polyclonal
Application:
IC, IF, IH, IP, WB
Reactivity:
H, M, R
Immunogen
Peptide TEEQKKYYNAMKKLGSKK(C), corresponding to amino acid residues 1501-1518 of rat NaV1.1 (Accession P04774). Intracellular loop between domains III and IV.
Homology
The epitope is identical in all isoforms of NaV1 in all vertebrates, and highly homologous in molluscan and insect voltage-gated Na+ channels.
Pan Nav Blocking Peptide Cat #: BLP-SC003
Size:
40 µg
Guinea pig Anti-Pan NaV Antibody Cat #: ASC-003-GP
Size:
50 µl
Host: Guinea pig
Type: Polyclonal
Application:
WB
Reactivity:
H, M, R
Immunogen
Peptide TEEQKKYYNAMKKLGSKK(C), corresponding to amino acid residues 1501-1518 of rat NaV1.1 (Accession P04774). Intracellular loop between domains III and IV.
Homology
The epitope is identical in all isoforms of NaV1 in all vertebrates, and highly homologous in molluscan and insect voltage-gated Na+ channels.
Pan Nav Blocking Peptide Cat #: BLP-SC003
Size:
40 µg
Anti-SCN1B (NaVβ1) (extracellular) Antibody Cat #: ASC-041
Size:
50 µl
Host: Rabbit
Type: Polyclonal
Application:
IC, IF, IH, LCI, WB
Reactivity:
M, R
Immunogen
Peptide CKRRSETTAETFTE, corresponding to amino acid residues 43-56 of rat β1 subunit of voltage-gated Na+ channels (Accession Q00954). Extracellular, N-terminus.
Homology
Mouse - identical; human -13/14 amino acid residues identical.
SCN1B/Navβ1 (extracellular) Blocking Peptide Cat #: BLP-SC041
Size:
40 µg
Anti-SCN2B (NaVβ2) Antibody Cat #: ASC-007
Size:
50 µl
Host: Rabbit
Type: Polyclonal
Application:
IF, IH, WB
Reactivity:
H, M, R
Immunogen
Peptide (C)KTEEEGKTDGEGNAEDGAK, corresponding to amino acid residues 197-215 of rat β2 subunit of voltage-gated Na+ channels (Accession P54900). Intracellular, C-terminus.
Homology
Human - 17/19 amino acid residues identical.
SCN2B/Navβ2 Blocking Peptide Cat #: BLP-SC007
Size:
40 µg
Anti-SCN4B (NaVβ4) (extracellular) Antibody Cat #: ASC-044
Size:
50 µl
Host: Rabbit
Type: Polyclonal
Application:
IC, IF, IFC, IH, LCI, WB
Reactivity:
H, M, R
Immunogen
Peptide (C)KNDKSDPKVRVKDD, corresponding to amino acid residues 85-98 of rat β4 subunit of voltage-gated Na+ channels (Accession Q7M730). Extracellular, N-terminus.
Homology
Mouse - identical; human - 11/14 amino acid residues identical.
SCN4B/Navβ4 (extracellular) Blocking Peptide Cat #: BLP-SC044
Size:
40 µg
Antibody Storage & Reconstitution Instructions
Storage before reconstitution
Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Reconstitution
Add deionized water, depending on the sample size.
Storage after reconstitution
The reconstituted solution can be stored at 4°C for up to 2 weeks. For longer periods, small aliquots should be stored at -20°C or below.
Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).
Control antigen storage before reconstitution
Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Control antigen reconstitution
100 μl water.
Control antigen storage after reconstitution
-20ºC.
Preadsorption control
1 μg peptide per 1 μg antibody.
Note
Guinea pig polyclonal antibodies (#ASC-005-GP, #ASC-016-GP, #ASC-017-GP and #ASC-003-GP) are included in this Explorer Kit. Please take into account when reacting with a secondary antibody.
For research purposes only, not for human use
