Cat #: AK-224_KIT
Size: 32 Vials
Form: Lyophilized
Application key:
CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot
Species reactivity key:
H- Human, M- Mouse, R- Rat
Anti-SCN1A (NaV1.1) Antibody Cat #: ASC-001
Size:
50 µl
Host: Rabbit
Type: Polyclonal
Application:
IC, IF, IH, IP, WB
Reactivity:
H, M, R
Immunogen
Peptide (C)TASEHSREPSAAGRLSD, corresponding to amino acid residues 465-481 of rat NaV1.1 (Accession P04774). Intracellular loop between domains I and II.
Homology
Mouse, human - identical.
SCN1A/Nav1.1 Blocking Peptide Cat #: BLP-SC001
Size:
40 µg
Guinea pig Anti-SCN1A (NaV1.1) Antibody Cat #: ASC-001-GP
Size:
50 µl
Host: Guinea pig
Type: Polyclonal
Application:
WB
Reactivity:
H, M, R
Immunogen
Peptide (C)TASEHSREPSAAGRLSD, corresponding to amino acid residues 465-481 of rat NaV1.1 (Accession P04774). Intracellular loop between domains I and II.
Homology
Mouse, human - identical.
SCN1A/Nav1.1 Blocking Peptide Cat #: BLP-SC001
Size:
40 µg
Anti-SCN2A (NaV1.2) Antibody Cat #: ASC-002
Size:
50 µl
Host: Rabbit
Type: Polyclonal
Application:
IC, IF, IH, IP, WB
Reactivity:
H, M, R
Immunogen
Peptide (C)ASAESRDFSGAGGIGVFSE, corresponding to amino acid residues 467-485 of rat NaV1.2 (Accession P04775). Intracellular loop between domains I and II.
Homology
Homology Mouse, Human - identical.
NaV1.3 – 12 out of 19 amino acid residues identical
NaV1.3 – 12 out of 19 amino acid residues identical
SCN2A/Nav1.2 Blocking Peptide Cat #: BLP-SC002
Size:
40 µg
Guinea pig Anti-SCN2A (NaV1.2) Antibody Cat #: ASC-002-GP
Size:
50 µl
Host: Guinea pig
Type: Polyclonal
Application:
IF, IH, WB
Reactivity:
H, M, R
Immunogen
Peptide (C)ASAESRDFSGAGGIGVFSE, corresponding to amino acid residues 467-485 of rat NaV1.2 (Accession P04775). Intracellular loop between domains I and II.
Homology
Human - identical.
NaV1.3 - 12/15 amino acid residues identical.
NaV1.3 - 12/15 amino acid residues identical.
SCN2A/Nav1.2 Blocking Peptide Cat #: BLP-SC002
Size:
40 µg
Anti-SCN3A (NaV1.3) Antibody Cat #: ASC-004
Size:
50 µl
Host: Rabbit
Type: Polyclonal
Application:
IC, IF, IH, IP, WB
Reactivity:
H, M, R
Immunogen
Peptide (C)HLEGNHRADGDRFP, corresponding to amino acid residues 511-524 rat NaV1.3 (Accession P08104). Intracellular, loop between domains I and II.
Homology
Mouse - 12/14 amino acid residues identical.
SCN3A/Nav1.3 Blocking Peptide Cat #: BLP-SC004
Size:
40 µg
Anti-SCN3A (NaV1.3) (extracellular) Antibody Cat #: ASC-023
Size:
50 µl
Host: Rabbit
Type: Polyclonal
Application:
IC, IF, LCI, WB
Reactivity:
R
Immunogen
Peptide CSQWPPSDSAFE, corresponding to amino acid residues 277-288 of rat NaV1.3 (Accession P08104). 3rd extracellular loop, domain I.
Homology
Mouse, human – 11/12 amino acid residues identical.
SCN3A/Nav1.3 (extracellular) Blocking Peptide Cat #: BLP-SC023
Size:
40 µg
Anti-SCN4A (NaV1.4) Antibody Cat #: ASC-020
Size:
50 µl
Host: Rabbit
Type: Polyclonal
Application:
IC, IF, WB
Reactivity:
H, M, R
Immunogen
Peptide EDKELKDNHILNHVG(C), corresponding to amino acid residues 877-891 of rat NaV1.4 (Accession P15390). Intracellular loop between domains II and III.
Homology
Mouse - 15/17 amino acid residues identical; human - 14/17 amino acid residues identical.
SCN4A/Nav1.4 Blocking Peptide Cat #: BLP-SC020
Size:
40 µg
Anti-NaV1.6 (SCN8A) Antibody Cat #: ASC-009
Size:
50 µl
Host: Rabbit
Type: Polyclonal
Application:
IC, IF, IH, WB
Reactivity:
H, M, R
Immunogen
Peptide CIANHTGVDIHRNGDFQKNG, corresponding to amino acid residues 1042-1061 of rat NaV1.6 (Accession O88420). Intracellular loop between domains II and III.
Homology
Mouse - identical; human - 19/20 amino acid residues identical.
Nav1.6/SCN8A Blocking Peptide Cat #: BLP-SC009
Size:
40 µg
Anti-NaV1.7 (SCN9A) Antibody Cat #: ASC-008
Size:
50 µl
Host: Rabbit
Type: Polyclonal
Application:
IC, IF, IH, WB
Reactivity:
H, M, R
Immunogen
Peptide (C)EFTSIGRSR IMGLSE, corresponding to amino acid residues 446-460 of rat NaV1.7 (Accession O08562). Intracellular loop between domains I and II.
Homology
Rabbit - 14/15 amino acid residues identical; human, mouse - 13/15 amino acid residues identical.
Nav1.7/SCN9A Blocking Peptide Cat #: BLP-SC008
Size:
40 µg
Guinea pig Anti-NaV1.7 (SCN9A) Antibody Cat #: ASC-008-GP
Size:
50 µl
Host: Guinea pig
Type: Polyclonal
Application:
WB
Reactivity:
R
Immunogen
Peptide (C)EFTSIGRSR IMGLSE, corresponding to amino acid residues 446-460 of rat NaV1.7 (Accession O08562). Intracellular loop between domains I and II.
Homology
Human, mouse - 13/15 amino acid residues identical.
Nav1.7/SCN9A Blocking Peptide Cat #: BLP-SC008
Size:
40 µg
Anti-NaV1.7 (SCN9A) (extracellular) Antibody Cat #: ASC-027
Size:
50 µl
Host: Rabbit
Type: Polyclonal
Application:
IC, IF, LCI, WB
Reactivity:
R
Immunogen
Peptide (C)EKEGQTEYMDY(K), corresponding to amino acid residues 1533-1543 of rat NaV1.7 (Accession O08562). 1st extracellular loop, domain IV.
Homology
Mouse - 8/11 amino acid residues identical.
Human - Not recommended to use with human samples.
Human - Not recommended to use with human samples.
Nav1.7/SCN9A (extracellular) Blocking Peptide Cat #: BLP-SC027
Size:
40 µg
Anti-Pan NaV Antibody Cat #: ASC-003
Size:
50 µl
Host: Rabbit
Type: Polyclonal
Application:
IC, IF, IH, IP, WB
Reactivity:
H, M, R
Immunogen
Peptide TEEQKKYYNAMKKLGSKK(C), corresponding to amino acid residues 1501-1518 of rat NaV1.1 (Accession P04774). Intracellular loop between domains III and IV.
Homology
The epitope is identical in all isoforms of NaV1 in all vertebrates, and highly homologous in molluscan and insect voltage-gated Na+ channels.
Pan Nav Blocking Peptide Cat #: BLP-SC003
Size:
40 µg
Guinea pig Anti-Pan NaV Antibody Cat #: ASC-003-GP
Size:
50 µl
Host: Guinea pig
Type: Polyclonal
Application:
WB
Reactivity:
H, M, R
Immunogen
Peptide TEEQKKYYNAMKKLGSKK(C), corresponding to amino acid residues 1501-1518 of rat NaV1.1 (Accession P04774). Intracellular loop between domains III and IV.
Homology
The epitope is identical in all isoforms of NaV1 in all vertebrates, and highly homologous in molluscan and insect voltage-gated Na+ channels.
Pan Nav Blocking Peptide Cat #: BLP-SC003
Size:
40 µg
Anti-SCN1B (NaVβ1) (extracellular) Antibody Cat #: ASC-041
Size:
50 µl
Host: Rabbit
Type: Polyclonal
Application:
IC, IF, IH, LCI, WB
Reactivity:
M, R
Immunogen
Peptide CKRRSETTAETFTE, corresponding to amino acid residues 43-56 of rat β1 subunit of voltage-gated Na+ channels (Accession Q00954). Extracellular, N-terminus.
Homology
Mouse - identical; human -13/14 amino acid residues identical.
SCN1B/Navβ1 (extracellular) Blocking Peptide Cat #: BLP-SC041
Size:
40 µg
Anti-SCN2B (NaVβ2) Antibody Cat #: ASC-007
Size:
50 µl
Host: Rabbit
Type: Polyclonal
Application:
IF, IH, WB
Reactivity:
H, M, R
Immunogen
Peptide (C)KTEEEGKTDGEGNAEDGAK, corresponding to amino acid residues 197-215 of rat β2 subunit of voltage-gated Na+ channels (Accession P54900). Intracellular, C-terminus.
Homology
Human - 17/19 amino acid residues identical.
SCN2B/Navβ2 Blocking Peptide Cat #: BLP-SC007
Size:
40 µg
Anti-SCN4B (NaVβ4) (extracellular) Antibody Cat #: ASC-044
Size:
50 µl
Host: Rabbit
Type: Polyclonal
Application:
IC, IF, IFC, IH, LCI, WB
Reactivity:
H, M, R
Immunogen
Peptide (C)KNDKSDPKVRVKDD, corresponding to amino acid residues 85-98 of rat β4 subunit of voltage-gated Na+ channels (Accession Q7M730). Extracellular, N-terminus.
Homology
Mouse - identical; human - 11/14 amino acid residues identical.
SCN4B/Navβ4 (extracellular) Blocking Peptide Cat #: BLP-SC044
Size:
40 µg
Antibody Storage & Reconstitution Instructions
Storage before reconstitution
Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Reconstitution
Add deionized water, depending on the sample size.
Storage after reconstitution
The reconstituted solution can be stored at 4°C for up to 2 weeks. For longer periods, small aliquots should be stored at -20°C or below.
Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).
Control antigen storage before reconstitution
Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Control antigen reconstitution
100 μl water.
Control antigen storage after reconstitution
-20ºC.
Preadsorption control
1 μg peptide per 1 μg antibody.
Note
Guinea pig polyclonal antibodies (#ASC-001-GP, #ASC-002-GP, #ASC-008-GP & #ASC-003-GP) are included in this Explorer Kit. Please take into account when reacting with a secondary antibody.
For research purposes only, not for human use
