Cat #: AK-224_KIT
Size: 32 Vials
Form: Lyophilized
Anti-SCN1A (NaV1.1) Antibody
Cat #: ASC-001
Size:
50 µl
Host: Rabbit
Type: Polyclonal
Application:
IC, IF, IH, IP, WB
Reactivity:
H, M, R
Immunogen
Peptide (C)TASEHSREPSAAGRLSD, corresponding to amino acid residues 465-481 of rat NaV1.1 (Accession P04774). Intracellular loop between domains I and II.
Homology
Mouse, human - identical.
SCN1A/Nav1.1 Blocking Peptide
Cat #: BLP-SC001
Guinea pig Anti-SCN1A (NaV1.1) Antibody
Cat #: ASC-001-GP (formerly AGP-043)
Size:
50 µl
Host: Guinea pig
Type: Polyclonal
Application:
WB
Reactivity:
H, M, R
Immunogen
Peptide (C)TASEHSREPSAAGRLSD, corresponding to amino acid residues 465-481 of rat NaV1.1 (Accession P04774). Intracellular loop between domains I and II.
Homology
Mouse, human - identical.
SCN1A/Nav1.1 Blocking Peptide
Cat #: BLP-SC001
Anti-SCN2A (NaV1.2) Antibody
Cat #: ASC-002
Size:
50 µl
Host: Rabbit
Type: Polyclonal
Application:
IC, IF, IH, IP, WB
Reactivity:
H, M, R
Immunogen
Peptide (C)ASAESRDFSGAGGIGVFSE, corresponding to amino acid residues 467-485 of rat NaV1.2 (Accession P04775). Intracellular loop between domains I and II.
Homology
Homology Mouse, Human - identical.
NaV1.3 – 12 out of 19 amino acid residues identical
SCN2A/Nav1.2 Blocking Peptide
Cat #: BLP-SC002
Guinea pig Anti-SCN2A (NaV1.2) Antibody
Cat #: ASC-002-GP (formerly AGP-026)
Size:
50 µl
Host: Guinea pig
Type: Polyclonal
Application:
IF, IH, WB
Reactivity:
H, M, R
Immunogen
Peptide (C)ASAESRDFSGAGGIGVFSE, corresponding to amino acid residues 467-485 of rat NaV1.2 (Accession P04775). Intracellular loop between domains I and II.
Homology
Human - identical.
NaV1.3 - 12/15 amino acid residues identical.
SCN2A/Nav1.2 Blocking Peptide
Cat #: BLP-SC002
Anti-SCN3A (NaV1.3) Antibody
Cat #: ASC-004
Size:
50 µl
Host: Rabbit
Type: Polyclonal
Application:
IC, IF, IH, IP, WB
Reactivity:
H, M, R
Immunogen
Peptide (C)HLEGNHRADGDRFP, corresponding to amino acid residues 511-524 rat NaV1.3 (Accession P08104). Intracellular, loop between domains I and II.
Homology
Mouse - 12/14 amino acid residues identical.
SCN3A/Nav1.3 Blocking Peptide
Cat #: BLP-SC004
Anti-SCN3A (NaV1.3) (extracellular) Antibody
Cat #: ASC-023
Size:
50 µl
Host: Rabbit
Type: Polyclonal
Application:
IC, IF, LCI, WB
Reactivity:
R
Immunogen
Peptide CSQWPPSDSAFE, corresponding to amino acid residues 277-288 of rat NaV1.3 (Accession P08104). 3rd extracellular loop, domain I.
Homology
Mouse, human – 11/12 amino acid residues identical.
SCN3A/Nav1.3 (extracellular) Blocking Peptide
Cat #: BLP-SC023
Anti-SCN4A (NaV1.4) Antibody
Cat #: ASC-020
Size:
50 µl
Host: Rabbit
Type: Polyclonal
Application:
IC, IF, WB
Reactivity:
H, M, R
Immunogen
Peptide EDKELKDNHILNHVG(C), corresponding to amino acid residues 877-891 of rat NaV1.4 (Accession P15390). Intracellular loop between domains II and III.
Homology
Mouse - 15/17 amino acid residues identical; human - 14/17 amino acid residues identical.
SCN4A/Nav1.4 Blocking Peptide
Cat #: BLP-SC020
Anti-NaV1.6 (SCN8A) Antibody
Cat #: ASC-009
Size:
50 µl
Host: Rabbit
Type: Polyclonal
Application:
IC, IF, IH, WB
Reactivity:
H, M, R
Immunogen
Peptide CIANHTGVDIHRNGDFQKNG, corresponding to amino acid residues 1042-1061 of rat NaV1.6 (Accession O88420). Intracellular loop between domains II and III.
Homology
Mouse - identical; human - 19/20 amino acid residues identical.
Nav1.6/SCN8A Blocking Peptide
Cat #: BLP-SC009
Anti-NaV1.7 (SCN9A) Antibody
Cat #: ASC-008
Size:
50 µl
Host: Rabbit
Type: Polyclonal
Application:
IC, IF, IH, WB
Reactivity:
H, M, R
Immunogen
Peptide (C)EFTSIGRSR IMGLSE, corresponding to amino acid residues 446-460 of rat NaV1.7 (Accession O08562). Intracellular loop between domains I and II.
Homology
Rabbit - 14/15 amino acid residues identical; human, mouse - 13/15 amino acid residues identical.
Nav1.7/SCN9A Blocking Peptide
Cat #: BLP-SC008
Guinea pig Anti-NaV1.7 (SCN9A) Antibody
Cat #: ASC-008-GP (formerly AGP-057)
Size:
50 µl
Host: Guinea pig
Type: Polyclonal
Application:
WB
Reactivity:
R
Immunogen
Peptide (C)EFTSIGRSR IMGLSE, corresponding to amino acid residues 446-460 of rat NaV1.7 (Accession O08562). Intracellular loop between domains I and II.
Homology
Human, mouse - 13/15 amino acid residues identical.
Nav1.7/SCN9A Blocking Peptide
Cat #: BLP-SC008
Anti-NaV1.7 (SCN9A) (extracellular) Antibody
Cat #: ASC-027
Size:
50 µl
Host: Rabbit
Type: Polyclonal
Application:
IC, IF, LCI, WB
Reactivity:
R
Immunogen
Peptide (C)EKEGQTEYMDY(K), corresponding to amino acid residues 1533-1543 of rat NaV1.7 (Accession O08562). 1st extracellular loop, domain IV.
Homology
Mouse - 8/11 amino acid residues identical.
Human - Not recommended to use with human samples.
Nav1.7/SCN9A (extracellular) Blocking Peptide
Cat #: BLP-SC027
Anti-Pan NaV Antibody
Cat #: ASC-003
Size:
50 µl
Host: Rabbit
Type: Polyclonal
Application:
IC, IF, IH, IP, WB
Reactivity:
H, M, R
Immunogen
Peptide TEEQKKYYNAMKKLGSKK(C), corresponding to amino acid residues 1501-1518 of rat NaV1.1 (Accession P04774). Intracellular loop between domains III and IV.
Homology
The epitope is identical in all isoforms of NaV1 in all vertebrates, and highly homologous in molluscan and insect voltage-gated Na+ channels.
Pan Nav Blocking Peptide
Cat #: BLP-SC003
Guinea pig Anti-Pan NaV Antibody
Cat #: ASC-003-GP (formerly AGP-007)
Size:
50 µl
Host: Guinea pig
Type: Polyclonal
Application:
WB
Reactivity:
H, M, R
Immunogen
Peptide TEEQKKYYNAMKKLGSKK(C), corresponding to amino acid residues 1501-1518 of rat NaV1.1 (Accession P04774). Intracellular loop between domains III and IV.
Homology
The epitope is identical in all isoforms of NaV1 in all vertebrates, and highly homologous in molluscan and insect voltage-gated Na+ channels.
Pan Nav Blocking Peptide
Cat #: BLP-SC003
Anti-SCN1B (NaVβ1) (extracellular) Antibody
Cat #: ASC-041
Size:
50 µl
Host: Rabbit
Type: Polyclonal
Application:
IC, IF, IH, LCI, WB
Reactivity:
M, R
Immunogen
Peptide CKRRSETTAETFTE, corresponding to amino acid residues 43-56 of rat β1 subunit of voltage-gated Na+ channels (Accession Q00954). Extracellular, N-terminus.
Homology
Mouse - identical; human -13/14 amino acid residues identical.
SCN1B/Navβ1 (extracellular) Blocking Peptide
Cat #: BLP-SC041
Anti-SCN2B (NaVβ2) Antibody
Cat #: ASC-007
Size:
50 µl
Host: Rabbit
Type: Polyclonal
Application:
IF, IH, WB
Reactivity:
H, M, R
Immunogen
Peptide (C)KTEEEGKTDGEGNAEDGAK, corresponding to amino acid residues 197-215 of rat β2 subunit of voltage-gated Na+ channels (Accession P54900). Intracellular, C-terminus.
Homology
Human - 17/19 amino acid residues identical.
SCN2B/Navβ2 Blocking Peptide
Cat #: BLP-SC007
Anti-SCN4B (NaVβ4) (extracellular) Antibody
Cat #: ASC-044
Size:
50 µl
Host: Rabbit
Type: Polyclonal
Application:
IC, IF, IFC, IH, LCI, WB
Reactivity:
H, M, R
Immunogen
Peptide (C)KNDKSDPKVRVKDD, corresponding to amino acid residues 85-98 of rat β4 subunit of voltage-gated Na+ channels (Accession Q7M730). Extracellular, N-terminus.
Homology
Mouse - identical; human - 11/14 amino acid residues identical.
SCN4B/Navβ4 (extracellular) Blocking Peptide
Cat #: BLP-SC044
Antibody Storage & Reconstitution Instructions
Storage before reconstitution
Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Reconstitution
Add deionized water, depending on the sample size.
Storage after reconstitution
The reconstituted solution can be stored at 4°C for up to 2 weeks. For longer periods, small aliquots should be stored at -20°C or below.
Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).
Control antigen storage before reconstitution
Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Control antigen reconstitution
100 μl water.
Control antigen storage after reconstitution
-20ºC.
Preadsorption control
1 μg peptide per 1 μg antibody.
Note
Guinea pig polyclonal antibodies (#ASC-001-GP, #ASC-002-GP, #ASC-008-GP & #ASC-003-GP) are included in this Explorer Kit. Please take into account when reacting with a secondary antibody.
For research purposes only, not for human use