Overview
- Kovalenko, M. et al. (1994) Cancer Res. 54, 6106.
- Milenkovic, I. et al. (2003) Invest. Ophthalmol. Vis. Sci. 44,1211.
- Iwasaki, H. et al. (1999) Endocrinology 140, 4659.
- Tse, K.F. et al. (2001) Leukemia 15, 1001.
- Matthews, W. et al. (1991) Cell 65,1143.
Protect from light.
Alomone Labs Tyrphostin AG 1296 inhibits the kinase activity of PDGF receptor in 3T3-L1 cells.Cells were serum starved for 3 h. The cells were then incubated for 30 min with various concentrations of Tyrphostin AG 1296 (#T-770) and stimulated with 10 ng/ml PDGF-BB. Cell proteins were resolved by SDS-PAGE and probed with anti-phospho-PDGFR (upper panel) and with antiphospho ERK1/2 (lower panel). Inhibition of ERK1/2 phosphorylation was dose-dependent.
- Kovalenko, M. et al. (1994) Cancer Res. 54, 6106.
- Milenkovic, I. et al. (2003) Invest. Ophthalmol. Vis. Sci. 44,1211.
- Iwasaki, H. et al. (1999) Endocrinology, 140, 4659.
- Levitzki, A. and Gazit, A. (1995) Science 267, 1782.
- Rice, A.B. et al. (1999) Am. J. Pathol. 155, 213.
- Krystal, G.W. et al. (1997) Cancer Res. 57, 2203.
- Tse, K.F. et al. (2001) Leukemia 15, 1001.
- Matthews, W. et al. (1991) Cell 65, 1143.
Tyrphostin AG1296 is a tyrosine kinase blocker which selectively inhibits platelet-derived growth factor (PDGF) a- and b-receptor kinases and the PDGF-dependent signaling in many cell types. Tyrphostin AG1296 also inhibits the PDGF dependent transactivation of the P2Y and Endothelin receptors-evoked cell proliferation and MAPK activation.1-4
This PDGF receptor (PDGFR) blocker has no effect on epidermal growth factor (EGF) receptor autophosphorylation; weak effects on DNA synthesis stimulated by insulin, by EGF, or by a combination of both; and over an order of magnitude weaker blocking effect on fibroblast growth factor-dependent (FGF) DNA synthesis.5-8 Tyrphostin AG1296 also potently inhibits the signaling of stem cell factor (SCF) receptor (c-Kit) and its upstream effector Kit receptor tyrosine kinase and the tyrosine kinase activity of FLT3-ITD which both have a strong sequence similarity with other members of the class III receptor tyrosine kinase but has no effect on the autophosphorylation of vascular endothelial growth factor (VEGF) receptor. 7,8
Tyrphostin AG 1296 (#T-770) is a highly pure, synthetic, and biologically active compound.
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