Overview
Type: Synthetic peptide
Form: Lyophilized powder
UCP2 Blocking Peptide (#BLP-NT128) is the original antigen used for immunization during Anti-UCP2 Antibody (#ANT-128) generation. The blocking peptide binds and ‘blocks’ Anti-UCP2 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of mouse heart (lanes 1 and 5), rat skeletal muscle (lanes 2 and 6), rat adipose tissue (lanes 3 and 7) and rat pancreas (lanes 4 and 8):1-4. Anti-UCP2 Antibody (#ANT-128), (1:200).
5-8. Anti-UCP2 Antibody, preincubated with UCP2 Blocking Peptide (#BLP-NT128).
Western blot analysis of mouse muscle myoblast (C2C12), human brain glioblastoma (U-87 MG), human colorectal adenocarcinoma (HT-29) cell lines:1-3. Anti-UCP2 Antibody (#ANT-128), (1:200).
4-6. Anti-UCP2 Antibody, preincubated with UCP2 Blocking Peptide (#BLP-NT128).
Expression of UCP2 in mouse hypothalamus.Immunohistochemical staining of perfusion-fixed frozen mouse brain sections with Anti-UCP2 Antibody (#ANT-128), (1:200), followed by goat anti-rabbit-AlexaFluor-488. A. UCP2 immunoreactivity (green) appears in neuron outlines (arrows). B. Pre-incubation of the antibody with UCP2 Blocking Peptide (#BLP-NT128), suppressed staining. Cell nuclei are stained with DAPI (blue). 3rd V = 3rd ventricle.
Expression of UCP2 in rat hypothalamus.Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-UCP2 Antibody (#ANT-128), (1:200), followed by goat anti-rabbit-AlexaFluor-488. A. UCP2 immunoreactivity (green) appears in neuron outlines (arrows). B. Pre-incubation of the antibody with UCP2 Blocking Peptide (#BLP-NT128), suppressed staining. Cell nuclei are stained with DAPI (blue). 3rd V = 3rd ventricle.
Properties
Sequence
- (C)GRRYQSTVEAYK, corresponding to amino acid residues 153-164 of rat mitochondrial uncoupling protein 2 (UCP2) (Accession P56500).
Accession (Uniprot) Number P56500
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
