Overview
Type: Synthetic peptide
Form: Lyophilized powder
Vasopressin V1A Receptor/AVPR1A Blocking Peptide (#BLP-VR010) is the original antigen used for immunization during Anti-Vasopressin V1A Receptor (AVPR1A) Antibody (#AVR-010) generation. The blocking peptide binds and ‘blocks’ Anti-Vasopressin V1A Receptor/AVPR1A primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat kidney (lanes 1 and 4), rat pancreas (lanes 2 and 5) and mouse heart (lanes 3 and 6) lysates:1-3. Anti-Vasopressin V1A Receptor (AVPR1A) Antibody (#AVR-010), (1:400).
4-6. Anti-Vasopressin V1A Receptor (AVPR1A) Antibody, preincubated with Vasopressin V1A Receptor/AVPR1A Blocking Peptide (#BLP-VR010).
Western blot analysis of human Burkitt's lymphoma (Daudi) cell line:1. Anti-Vasopressin V1A Receptor (AVPR1A) Antibody (#AVR-010), (1:400).
2. Anti-Vasopressin V1A Receptor (AVPR1A) Antibody, preincubated with Vasopressin V1A Receptor/AVPR1A Blocking Peptide (#BLP-VR010).
Expression of Vasopressin V1A Receptor in rat horizontal diagonal band.Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-Vasopressin V1A Receptor (AVPR1A) Antibody (#AVR-010), (1:200), followed by goat anti-rabbit-AlexaFluor-488. A. Vasopressin V1A Receptor immunoreactivity (green) appears in outlines of neurons (arrows). B. Pre-incubation of the antibody with Vasopressin V1A Receptor (AVPR1A) Blocking Peptide (BLP-VR010), suppressed staining. Cell nuclei are stained with DAPI (blue).
Expression of Vasopressin V1A Receptor in rat frontal cortex.Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-Vasopressin V1A Receptor (AVPR1A) Antibody (#AVR-010), (1:200), followed by goat anti-rabbit-AlexaFluor-488. A. Vasopressin V1A Receptor immunoreactivity (green) appears in outlines of cortical neurons (arrows). B. Pre-incubation of the antibody with Vasopressin V1A Receptor (AVPR1A) Blocking Peptide (BLP-VR010), suppressed staining. Cell nuclei are stained with DAPI (blue).
Western blot analysis of rat brain membranes (lanes 1 and 3) and mouse brain membranes (lanes 2 and 4):1, 2. Guinea Pig Anti-Vasopressin V1A Receptor (AVPR1A) Antibody (#AVR-010-GP), (1:200).
3, 4. Guinea Pig Anti-Vasopressin V1A Receptor (AVPR1A) Antibody, preincubated with Vasopressin V1A Receptor (AVPR1A) Blocking Peptide (#BLP-VR010).
Western blot analysis of rat kidney membranes (lanes 1 and 3) and mouse kidney membranes (lanes 2 and 4):1, 2. Guinea Pig Anti-Vasopressin V1A Receptor (AVPR1A) Antibody (#AVR-010-GP), (1:200).
3, 4. Guinea Pig Anti-Vasopressin V1A Receptor (AVPR1A) Antibody, preincubated with Vasopressin V1A Receptor (AVPR1A) Blocking Peptide (#BLP-VR010).
Expression of V1aR in rat medial septum.Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Guinea Pig Anti-Vasopressin V1A Receptor (AVPR1A) Antibody (#AVR-010-GP), (1:200), followed by goat anti-guinea pig - Alexa Fluor-594. A. V1aR immunoreactivity (red) appears in neuronal outlines (arrows). B. Pre-incubation of the antibody with Vasopressin V1A Receptor (AVPR1A) Blocking Peptide (#BLP-VR010), suppressed staining. Cell nuclei are stained with DAPI (blue).
Properties
Sequence
- (C)KFAKDDSDSMSRR, corresponding to amino acid residues 378-390 of rat AVPR1A (Accession P30560).
Accession (Uniprot) Number P30560
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
