Overview
Alternative Name Calan®, Verelan®, Isoptin®, Covera-HS®
Lyophilized Powder yes
Source Synthetic
MW: 491.1
Purity: >99%.
Effective concentration 1-250 ?M.
Structure
Chemical name α-[3-[[2-(3,4-Dimethoxyphenyl)ethyl]methylamino]propyl]-3,4-dimethoxy-α-(1-methylethyl)benzeneacetonitrile hydrochloride.
Molecular formula C27H38N2O4*HCl.
CAS No.: 152-11-4
Activity Verapamil HCl is a phenylalkylamine that acts as an L-type, voltage-gated Ca2+ channel blocker and is used in the clinic to treat hypertension1,2. Verapamil block of L-type channels is dependent on the membrane potential. The IC50 values at a holding potential of -80 or -40 mV are 250 and 15.5 μM, respectively3. In Xenopus oocytes, Verapamil inhibits L-type currents in an activity dependent manner4.
References-Activity
- Zanchetti, A. et al. (1998) J. Hypertension 16, 1667.
- Hockerman, G. H. et al. (1997) Annu. Rev. Pharmacol. Toxicol. 37, 361.
- Lacinova, L. et al. (1995) J. Pharmacol. Exp. Ther. 274, 54.54.
- Huber, I.G. et al. (2004) J. Biol. Chem. 279, 55211.
Shipping and storage Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Solubility Up to 50mM in water, DMSO and other solvents. Mild agitation and/or warming my help dissolve the product at higher stock concentrations. Centrifuge all product preparations before opening/handling (10000 x g 5 min).
Storage of solutions Up to four week at 4°C or three months at -20°C.
Our bioassay
Alomone Labs Verapamil HCl inhibits L-type Ca2+ currents in Xenopus oocytes.A. Time course of L-type channel (CaV1.2+α2δ1+β1a) activity before and during applications of 10 and100 μM Verapamil HCl (#V-100) and upon wash (Verapamil HCl application periods are indicated by the horizontal bars). Holding potential was -100 mV and currents were elicited every 10 seconds by 100 ms steps to -10 mV. X axis in minutes and Y axis in nA. B. Example current traces before and during applications of 10 and 100 μM Verapamil HCl (concentrations indicated are taken from the experiment described in A).
Target L-type Ca2+ channels
Lyophilized Powder
For research purposes only, not for human use
Last Update: 30/03/2021
Specifications
Citations
Citations
