Cat #: BLP-CT003
Size: 40 µg
Type: Synthetic peptide
Form: Lyophilized powder
Applications: wb, ihc
Application key:
WB- Western blot, IHC- Immunohistochemistry
For research purposes only. not for human use
Properties
Sequence
- (C)DAVRLREVQGKDGGE, corresponding to amino acid residues 495-509 of rat VAChT (Accession Q62666).
Accession (Uniprot) Number Q62666
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 2 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
Applications
Demonstration of Pre-adsorption control
- Western blot analysis of mouse (lanes 1 and 3) and rat (lanes 2 and 4) brain lysates:1,2. Anti-Vesicular Acetylcholine Transporter (VAChT) Antibody (#ACT-003), (1:200).
3,4. Anti-Vesicular Acetylcholine Transporter (VAChT) Antibody, preincubated with Vesicular Acetylcholine Transporter/VACHT Blocking Peptide (#BLP-CT003). - Rat brain and spinal cord sections (1:400).
Last update: 02/01/2024