Overview
Type: Synthetic peptide
Form: Lyophilized powder
Vesicular GABA Transporter/VGAT Blocking Peptide (#BLP-GT005) is the original antigen used for immunization during Anti-Vesicular GABA Transporter (VGAT) Antibody (#AGT-005) generation. The blocking peptide binds and ‘blocks’ Anti-Vesicular GABA Transporter/VGAT primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat brain membranes (lanes 1 and 4), mouse brain membranes (lanes 2 and 5) and human U-87 MG glyoblastoma lysates (lanes 3 and 6):1-3. Anti-Vesicular GABA Transporter (VGAT) Antibody (#AGT-005), (1:200).
4-6. Anti-Vesicular GABA Transporter (VGAT) Antibody, preincubated with Vesicular GABA Transporter/VGAT Blocking Peptide (#BLP-GT005).
Expression of Vesicular GABA Transporter in rat cerebellumImmunohistochemical staining of rat frozen cerebellum sections using Anti-Vesicular GABA Transporter (VGAT) Antibody (#AGT-005), (1:100). VGAT staining is shown in red and dapi (blue) was used as a general cellular marker. VGAT appears in the cerebellar pinceau (arrows) and in the soma of Purkinje cells (triangles).
Western blot analysis of rat brain membranes (lanes 1 and 5), mouse brain membranes (lanes 2 and 6), human U-87 MG glyoblastoma lysates (lanes 3 and 7) and human SH-SY5Y brain neuroblastoma lysates (lanes 4 and 8):1-4. Guinea pig Anti-Vesicular GABA Transporter (VGAT) Antibody (#AGT-005-GP), (1:200).
5-8. Guinea pig Anti-Vesicular GABA Transporter (VGAT) Antibody, preincubated with Vesicular GABA Transporter/VGAT Blocking Peptide (#BLP-GT005).
Expression of VGAT in mouse cerebellum.Immunohistochemical staining of perfusion-fixed frozen mouse brain sections with Guinea pig Anti-Vesicular GABA Transporter (VGAT) Antibody (#AGT-005-GP), (1:600), followed by goat anti-guinea pig-AlexaFluor-594. A. VGAT immunoreactivity (red) appears in the granule layer (GL) and in “pinceau” structures adjacent to purkinje cells (arrows). B. Pre-incubation of the antibody with Vesicular GABA Transporter/VGAT Blocking Peptide (#BLP-GT005), suppressed staining. Cell nuclei are stained with DAPI (blue).
Expression of VGAT in rat cerebellum.Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Guinea pig Anti-Vesicular GABA Transporter (VGAT) Antibody (#AGT-005-GP), (1:600), followed by goat anti-guinea pig-AlexaFluor-594. A. VGAT immunoreactivity (red) appears in the granule layer (GL) and in “pinceau” structures adjacent to purkinje cells (arrows). B. Pre-incubation of the antibody with Vesicular GABA Transporter/VGAT Blocking Peptide (#BLP-GT005), suppressed staining. Cell nuclei are stained with DAPI (blue).
Properties
Sequence
- (C)GEFGGHDKPKITAWE, corresponding to amino acid residues 106-120 of rat vesicular GABA transporter (Accession O35458).
Accession (Uniprot) Number O35458
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
