Size: 40 µg
Type: Synthetic peptide
Form: Lyophilized powder
Applications: wb, ihc
For research purposes only. not for human use
Properties
Sequence
- (C)EFRQEEFRKLAGR, corresponding to amino acid residues 2-14 of rat VGLUT1 (Accession Q62634).
Accession (Uniprot) Number Q62634
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat cortex lysate (lanes 1 and 3) and mouse brain lysate (lanes 2 and 4):1,2. Anti-VGLUT1 Antibody (#AGC-035), (1:200).
3,4. Anti-VGLUT1 Antibody, preincubated with VGLUT1 Blocking Peptide (#BLP-GC035).- Rat brain sections (1:400).
Western blot analysis of rat brain cortex lysate (lanes 1 and 3) and mouse brain lysate (lanes 2 and 4):1-2. Guinea Pig Anti-VGLUT1 Antibody (#AGC-035-GP), (1:400).
3-4. Guinea Pig Anti-VGLUT1 Antibody, preincubated with VGLUT1 Blocking Peptide (#BLP-GC035).
Last update: 18/02/2024
