Overview
Type: Synthetic peptide
Form: Lyophilized powder
VMAT2 Blocking Peptide (#BLP-MT006) is the original antigen used for immunization during Anti-VMAT2 Antibody (#AMT-006) generation. The blocking peptide binds and ‘blocks’ Anti-VMAT2 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat brain (lanes 1 and 4), mouse brain (lanes 2 and 5) and rat brain stem (lanes 3 and 6) lysates:1-3. Anti-VMAT2 Antibody (#AMT-006), (1:400).
4-6. Anti-VMAT2 Antibody, preincubated with VMAT2 Blocking Peptide (#BLP-MT006).
Expression of VMAT2 in rat substantia nigra dopaminergic neuronsImmunohistochemical staining of perfusion-fixed frozen rat brain sections using Anti-VMAT2 Antibody (#AMT-006), (1:1000). A. VMAT2 staining (red). B. Tyrosine hydroxylase staining (green), a marker of dopaminergic neurons. C. Merger of A and B demonstrates VMAT2 expression in several dopaminergic neurons (arrows). Cell nuclei were stained with DAPI (blue).
Properties
Sequence
- (C)KHEKNSTEIQT(A)R, corresponding to amino acid residues 52-64 of rat VMAT2 (Accession Q01827).
Accession (Uniprot) Number Q01827
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
