Overview
Alternative Name N-Ethyl-5-methyl-2-(1-methylethyl)cyclohexanecarboxamide, Ethyl menthane carboxamide, N-Ethyl-p-menthane-3-carboxamide, N-Ethyl-2-isopropyl-5-methylcyclohexanecarboxamide
Lyophilized Powder yes
Source Synthetic
MW: 211.34
Purity: >98%
Effective concentration 0.1-10 µM.
Structure
Chemical name N-ethyl-5-methyl-2-propan-2-ylcyclohexane-1-carboxamide.
Molecular formula C13H25NO.
CAS No.: 39711-79-0
PubChem CID 62907
Activity WS 3 is a TRPM8 channel activator (EC50 = 3.7 μM)1.
References-Activity
- Behrendt, H.J. et al. (2004) Br. J. Pharmacol. 141, 737.
Shipping and storage Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Solubility Up to 100mM in DMSO. Centrifuge all products before use (10000 x g 5 min).
Storage of solutions Up to three months at -20°C.
Our bioassay
Alomone Labs WS 3 activates TRPM8 channel expressed in HEK-293 cells.Dose response curve for the activation of human TRPM8 by increseaing WS 3 (#W-100) concentrations. EC50 is estimated at 0.43 µM. Cells were loaded with Calcium-5 dye. Changes in intracellular Ca2+ were detected via changes in 485 nm/525 nm fluorescence following agonist application.
References - Scientific background
- Bodding, M. et al. (2007) Cell Calcium 42, 618.
- Tsumura, M. et al. (2013) PLoS One 8, 12.
- Behrendt, H.J. et al. (2004) Br. J. Pharmacol. 141, 737.
Scientific background
WS 3 is a synthetic, selective TRPM8 channel agonist with an EC50 value of 3.7 μM1,3.
TRPM8 is a Ca2+-permeable channel, a member of the ‘long' or melastatin (M) group of TRP proteins and is activated by temperatures or by cooling compounds such as icilin, menthol and eucalyptol. The TRPM8 gene is expressed and is up-regulated in prostate cancer and other malignancies. It is specifically expressed in temperature-sensing trigeminal and dorsal root ganglion neurons1-3.
Target TRPM8 channel
Lyophilized Powder
WS 3 (#W-100) is a highly pure, synthetic, and biologically active compound.
For research purposes only, not for human use
Last Update: 07/05/2024
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