Size: 40 µg
Type: Synthetic peptide
Form: Lyophilized powder
Applications: wb, ihc
For research purposes only. not for human use
Properties
Sequence
- (C)THHFKDAFSGRDTS, corresponding to amino acid residues 218-231 of mouse cystine/glutamate transporter (Accession Q9WTR6).
Accession (Uniprot) Number Q9WTR6
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat brain (lanes 1 and 4), mouse brain (lanes 2 and 5) and rat stomach (lanes 3 and 6) lysates:1-3. Anti-xCT/SLC7A11 (extracellular) Antibody (#ANT-111), (1:800).
4-6. Anti-xCT/SLC7A11 (extracellular) Antibody, preincubated with xCT/SLC7A11 (extracellular) Blocking Peptide (#BLP-NT111).
Western blot analysis of human brain glioblastoma cell line (U-87 MG) lysate:1. Anti-xCT/SLC7A11 (extracellular) Antibody (1:200).
2. Anti-xCT/SLC7A11 (extracellular) Antibody, preincubated with xCT/SLC7A11 (extracellular) Blocking Peptide (#BLP-NT111).- Mouse brain sections (1:300).
Last update: 02/01/2024
