Overview
Type: Synthetic peptide
Form: Lyophilized powder
ZIP1/SLC39A1 (extracellular) Blocking Peptide (#BLP-ZT001) is the original antigen used for immunization during Anti-ZIP1 (SLC39A1) (extracellular) Antibody (#AZT-001) generation. The blocking peptide binds and ‘blocks’ Anti-ZIP1/SLC39A1 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain lysates:1,2. Anti-ZIP1 (SLC39A1) (extracellular) Antibody (#AZT-001), (1:200).
3,4. Anti-ZIP1 (SLC39A1) (extracellular) Antibody, preincubated with ZIP1/SLC39A1 (extracellular) Blocking Peptide (#BLP-ZT001).
Western blot analysis of human U-87 MG glioblastoma cell lysate (lanes 1 and 3) and human MDA-231 breast adenocarcinoma cell lysate (lanes 2 and 4):1,2. Anti-ZIP1 (SLC39A1) (extracellular) Antibody (#AZT-001), (1:200).
3,4. Anti-ZIP1 (SLC39A1) (extracellular) Antibody, preincubated with ZIP1/SLC39A1 (extracellular) Blocking Peptide (#BLP-ZT001).
Expression of ZIP1 in rat hippocampusImmunohistochemical staining of perfusion-fixed frozen rat brain sections using Anti-ZIP1 (SLC39A1) (extracellular) Antibody (#AZT-001), (1:400), followed by anti-rabbit-Alexa-488 antibody. ZIP1 is expressed (green) in the mossy fiber (MF) terminal field (arrows) of the CA3 hippocampal region. Nuclei are labeled with DAPI (blue).
Properties
Sequence
- (C)GEPELLVWRPEAVASE, corresponding to amino acid residues 5-20 of human ZIP1 (Accession Q9NY26).
Accession (Uniprot) Number Q9NY26
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
