Overview
Type: Synthetic peptide
Form: Lyophilized Powder
ZIP10/SLC39A10 (extracellular) Blocking Peptide (#BLP-ZT010) is the original antigen used for immunization during Anti-ZIP10/SLC39A10 (extracellular) Antibody (#AZT-010) generation. The blocking peptide binds and ‘blocks’ Anti-ZIP10/SLC39A10 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat brain membranes (lanes 1 and 3) and mouse brain membranes (lanes 2 and 4):1-2. Anti-ZIP10/SLC39A10 (extracellular) Antibody (#AZT-010), (1:200).
3-4. Anti-ZIP10/SLC39A10 (extracellular) Antibody, preincubated with ZIP10/SLC39A10 (extracellular) Blocking Peptide (BLP-ZT010).
Western blot analysis of human Jurkat T-cell leukemia cell line lysate (lanes 1 and 4), human THP-1 monocytic leukemia cell line lysate (lanes 2 and 5) and human K562 chronic myelogenous leukemia cell line lysate (lanes 3 and 6):1-3. Anti-ZIP10/SLC39A10 (extracellular) Antibody (#AZT-010), (1:200).
4-6. Anti-ZIP10/SLC39A10 (extracellular) Antibody, preincubated with ZIP10/SLC39A10 (extracellular) Blocking Peptide (BLP-ZT010).
Expression of ZIP10 in mouse hippocampusImmunohistochemical staining of perfusion-fixed frozen mouse brain sections with Anti-ZIP10/SLC39A10 (extracellular) Antibody (#AZT-010), (1:300), followed by goat anti-rabbit-AlexaFluor-488. A. Staining in the hippocampal CA3 region, showed ZIP10 immunoreactivity (green) in cells of the pyramidal layer (arrows). B. Pre-incubation of the antibody with ZIP10/SLC39A10 (extracellular) Blocking Peptide (BLP-ZT010), suppressed staining. Cell nuclei are stained with DAPI (blue). P = pyramidal layer, CA3-SR = CA3 stratum radiatum
Properties
Sequence
- Peptide (C)EQYEHNRVHKLDRVH, corresponding to amino acid residues 259 - 273 of mouse SLC39A10 (Accession Q6P5F6).
Accession (Uniprot) Number Q6P5F6
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
