Overview
Type: Synthetic peptide
Form: Lyophilized powder
ZIP3/SLC39A3 Blocking Peptide (#BLP-ZT003) is the original antigen used for immunization during Anti-ZIP3 (SLC39A3) Antibody (#AZT-003) generation. The blocking peptide binds and ‘blocks’ Anti-ZIP3/SLC39A3 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of mouse lung (lanes 1 and 4), rat brain (lanes 2 and 5) and mouse brain (lanes 3 and 6) lysates:1-3. Anti-ZIP3 (SLC39A3) Antibody (#AZT-003), (1:400).
4-6. Anti-ZIP3 (SLC39A3) Antibody, preincubated with ZIP3/SLC39A3 Blocking Peptide (#BLP-ZT003).
Western blot analysis of human MCF-7 breast adenocarcinoma cell lysate (lanes 1 and 3) and human LNCaP prostate adenocarcinoma cell lysate (lanes 2 and 4):1,2. Anti-ZIP3 (SLC39A3) Antibody (#AZT-003), (1:200).
3,4. Anti-ZIP3 (SLC39A3) Antibody, preincubated with ZIP3/SLC39A3 Blocking Peptide (#BLP-ZT003).
Expression of Zinc transporter ZIP3 in rat hippocampusImmunohistochemical staining of perfusion-fixed frozen rat brain sections using Anti-ZIP3 (SLC39A3) Antibody (#AZT-003), (1:400), followed by goat-anti-rabbit-AlexaFluor-488 antibody. ZIP3 staining (green) appears in CA1 neurons in the pyramidal layer (arrow). Nuclei are labeled with DAPI (blue).
Properties
Sequence
- (C)EADFEKAHRSKK, corresponding to amino acid residues 31-42 of rat ZIP3 (Accession Q5U1X7).
Accession (Uniprot) Number Q5U1X7
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
