Overview
Type: Synthetic peptide
Form: Lyophilized powder
ZIP8/SLC39A8 (extracellular) Blocking Peptide (#BLP-ZT008) is the original antigen used for immunization during Anti-ZIP8 (SLC39A8) (extracellular) Antibody (#AZT-008) generation. The blocking peptide binds and ‘blocks’ Anti-ZIP8/SLC39A8 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of mouse kidney (lanes 1 and 3) and mouse brain (lanes 2 and 4) lysates:1,2. Anti-ZIP8 (SLC39A8) (extracellular) Antibody (#AZT-008), (1:200).
3,4. Anti-ZIP8 (SLC39A8) (extracellular) Antibody, preincubated with ZIP8/SLC39A8 (extracellular) Blocking Peptide (#BLP-ZT008).
Western blot analysis of rat brain lysate:1. Anti-ZIP8 (SLC39A8) (extracellular) Antibody (#AZT-008), (1:200).
2. Anti-ZIP8 (SLC39A8) (extracellular) Antibody, preincubated with ZIP8/SLC39A8 (extracellular) Blocking Peptide (#BLP-ZT008).
Western blot analysis of human Jurkat T-cell leukemia cell line (lanes 1 and 3) and human K562 chronic myelogenous leukemia cell line (lanes 2 and 4) lysates:1,2. Anti-ZIP8 (SLC39A8) (extracellular) Antibody (#AZT-008), (1:200).
3,4. Anti-ZIP8 (SLC39A8) (extracellular) Antibody, preincubated with ZIP8/SLC39A8 (extracellular) Blocking Peptide (#BLP-ZT008).
Expression of Zinc transporter ZIP8 in rat hippocampusImmunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-ZIP8 (SLC39A8) (extracellular) Antibody (#AZT-008), (1:200), followed by goat-anti-rabbit-AlexaFluor-488. ZIP8 staining (green) appears in interneurons (arrows) adjacent to the granular layer (GL). Cell nuclei are stained with DAPI (blue).
Properties
Sequence
- (C)KTYGQNDHTHFRND, corresponding to amino acid residues 221-234 of rat ZIP8 (Accession Q5FVQ0).
Accession (Uniprot) Number Q5FVQ0
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
