Overview
Type: Synthetic peptide
Form: Lyophilized powder
ZnT1/SLC30A1 Blocking Peptide (#BLP-ZT011) is the original antigen used for immunization during Anti-ZnT1 (SLC30A1) Antibody (#AZT-011) generation. The blocking peptide binds and ‘blocks’ Anti-ZnT1/SLC30A1 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of mouse lung lysate (lanes 1 and 4), rat liver lysate (lanes 2 and 5) and rat brain membranes (lanes 3 and 6):1-3. Anti-ZnT1 (SLC30A1) Antibody (#AZT-011), (1:400).
4-6. Anti-ZnT1 (SLC30A1) Antibody, preincubated with ZnT1/SLC30A1 Blocking Peptide (#BLP-ZT011).
Expression of Zinc transporter 1 in rat olfactory bulbImmunohistochemical staining of perfusion-fixed frozen rat brain sections using Anti-ZnT1 (SLC30A1) Antibody (#AZT-011), (1:300), followed by goat anti-rabbit-AlexaFluor-488 antibody. ZnT1 staining (green) appears in glomeruli (G) and is particularly intense in the outer border (arrows). Cell nuclei are labeled with DAPI (blue).
Properties
Sequence
- (C)ENLEKKPRRTKAE, corresponding to amino acid residues 473-485 of rat ZnT1 (Accession Q62720).
Accession (Uniprot) Number Q62720
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
